The osteoregenerative effects of platelet-derived growth factor BB cotransplanted with mesenchymal stem cells, loaded on freeze-dried mineral bone block: A pilot study in dog mandible
Corresponding Author
Arash Khojasteh
Department of Oral and Maxillofacial Surgery, Research Institute of Dental Sciences, Dental school, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Correspondence to: A. Khojasteh; (e-mail: [email protected])Search for more papers by this authorSeyedeh Ghazaleh Dashti
Dental Research Center, Research Institute of Dental Sciences of Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMohammad Mehdi Dehghan
Department of Surgery and Radiology, Center of Excellence for Cell Therapy and Tissue Engineering, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Search for more papers by this authorHossein Behnia
Dental Research Center, Research Institute of Dental Sciences, Dental school, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorPegah Abbasnia
Department of Surgery and Radiology, Center of Excellence for Cell Therapy and Tissue Engineering, Faculty of Veterinary Medicine, The University of Tehran, Tehran, Iran
Search for more papers by this authorGolnaz Morad
Dental Research Center, Research Institute of Dental Sciences of Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorCorresponding Author
Arash Khojasteh
Department of Oral and Maxillofacial Surgery, Research Institute of Dental Sciences, Dental school, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Correspondence to: A. Khojasteh; (e-mail: [email protected])Search for more papers by this authorSeyedeh Ghazaleh Dashti
Dental Research Center, Research Institute of Dental Sciences of Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMohammad Mehdi Dehghan
Department of Surgery and Radiology, Center of Excellence for Cell Therapy and Tissue Engineering, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Search for more papers by this authorHossein Behnia
Dental Research Center, Research Institute of Dental Sciences, Dental school, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorPegah Abbasnia
Department of Surgery and Radiology, Center of Excellence for Cell Therapy and Tissue Engineering, Faculty of Veterinary Medicine, The University of Tehran, Tehran, Iran
Search for more papers by this authorGolnaz Morad
Dental Research Center, Research Institute of Dental Sciences of Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorFunding: This study was financially supported by Dental Research Center, Dental School, Shahid Beheshti University of Medical Science.
Abstract
Due to shortcomings associated with autogenous bone graft, the gold standard of craniofacial grafting, investigators seek alternatives that are accessible, efficient, and affordable. Accordingly, in the present pilot study, bone regeneration was induced using bone marrow-derived mesenchymal stem cells (BMSCs) loaded onto freeze-dried mineral bone block (FDMBB) in the presence or absence of recombinant platelet-derived growth factor-BB (rh PDGF-BB). Eight weeks after the bilateral extraction of premolars of four mongrel dogs, 25 × 10 mm defects were created at both sides of the mandible. The right mandible received autogenous-BMSC loaded on FDMBB (MSC group), whereas the left mandible received cellular blocks impregnated with rhPDGF-BB (MSC + PDGF Group). Animals were euthanized 8 weeks after grafting. Micro-computed tomography (micro-CT) and histomorphometric analysis demonstrated higher levels of bone formation for the test group (10.34% ± 0.20 and 26.63% ± 3.14, respectively) when compared to the control group (8.20% ± 0.20 and 21.38% ± 5.11). The differences were not statistically significant (P > 0.05). According to the performed micro-CT and histomorphometric analysis, adding 0.5 mg rhPDGF-BB (0.3 mg/mL) to the combination of BMSC/FDMBB did not significantly increase bone formation in supracrestal defect in dog mandible. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1771–1778, 2014.
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