Cytocompatible and water-stable camelina protein films for tissue engineering
Yi Zhao
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Search for more papers by this authorQiuran Jiang
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Search for more papers by this authorHelan Xu
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Search for more papers by this authorNarendra Reddy
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Search for more papers by this authorLan Xu
Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, Nabraska, 68583-0915
Search for more papers by this authorCorresponding Author
Yiqi Yang
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Nebraska Center for Materials and Nanoscience, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Correspondence to: Y. Yang (e-mail: [email protected])Search for more papers by this authorYi Zhao
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Search for more papers by this authorQiuran Jiang
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Search for more papers by this authorHelan Xu
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Search for more papers by this authorNarendra Reddy
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Search for more papers by this authorLan Xu
Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, Nabraska, 68583-0915
Search for more papers by this authorCorresponding Author
Yiqi Yang
Department of Textiles, Merchandising and Fashion Design, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Nebraska Center for Materials and Nanoscience, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0802
Correspondence to: Y. Yang (e-mail: [email protected])Search for more papers by this authorAbstract
In this research, films with compressive strength and aqueous stability were developed from camelina protein (CP) for tissue engineering. Protein based scaffolds have poor mechanical properties and aqueous stability and generally require chemical or physical modifications to make them applicable for medical applications. However, these modifications such as crosslinking could reduce biocompatibility and/or degradability of the scaffolds. Using proteins that are inherently water-stable could avoid modifications and provide scaffolds with the desired properties. CP with a high degree of disulfide cross-linkage has the potential to provide water-stable biomaterials, but it is difficult to dissolve CP and develop scaffolds. In this study, a new method of dissolving highly cross-linked proteins that results in limited hydrolysis and preserves the protein backbone was developed to produce water-stable films from CP without any modification. Only 12 % weight loss of camelina films was observed after 7 days in phosphate buffer saline (PBS) at 37°C. NIH 3T3 fibroblasts could attach and proliferate better on camelina films than on citric acid cross-linked collagen films. Therefore, CP films have the potential to be used for tissue engineering, and this extraction-dissolution method can be used for developing biomedical materials from various water-stable plant proteins. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 102B: 729–736, 2014.
REFERENCES
- 1 Jain D, Banerjee R. Comparison of ciprofloxacin hydrochloride-loaded protein, lipid, and chitosan nanoparticles for drug delivery. J Biomed Mater Res B Appl Biomater 2008; 86: 105–112.
- 2 MaHam A, Tang ZW, Wu H, Wang J, Lin YH. Protein-based nanomedicine platforms for drug delivery. Small 2009; 5: 1706–1721.
- 3 Chan G, Mooney DJ. New materials for tissue engineering: Towards greater control over the biological response. Trends Biotechnol 2008; 26: 382–392.
- 4 Chong EJ, Phan TT, Lim IJ, Zhang YZ, Bay BH, Ramakrishna S, Lim CT. Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomater 2007; 3: 321–330.
- 5 Reddy N, Jiang QR, Yang YQ. Novel wheat protein films as substrates for tissue engineering. J Biomater Sci Polym Ed 2011; 22: 2063–2077.
- 6 Jiang QR, Reddy N, Yang YQ. Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds. Acta Biomater 2010; 6: 4042–4051.
- 7 Pace CN, Trevino S, Prabhakaran E, Scholtz JM. Protein structure, stability and solubility in water and other solvents. Philos Trans R Soc London Ser B 2004; 359: 1225–1234.
- 8 Reddy N, Yang Y. Potential of plant proteins for medical applications. Trends Biotechnol 2011; 29: 490–498.
- 9 Huang L, Nagapudi K, Apkarian RP, Chaikof EL. Engineered collagen-PEO nanofibers and fabrics. J Biomater Sci Polym Ed 2001; 12: 979–993.
- 10 Zhang J, Mungara P, Jane J. Mechanical and thermal properties of extruded soy protein sheets. Polymer 2001; 42: 2569–2578.
- 11 Jin HJ, Park J, Karageorgious V, Kim UJ, Valluzzi R, Cebe P, Kaplan DL. Water stable silk film with reduced β sheet content. Adv Funct Mater 2005; 15: 1241–1247.
- 12
Lin L,
Perets A,
Har-el Y,
Varma D,
Li MY,
Lazarovici P,
Woerdeman DL,
Lelkes PI. Alimentary‘green' proteins as electrospun scaffolds for skin regenerative engineering. J Tissue Eng Regen Med 2012; DOI: 10.1002/term.1493.
10.1002/term.1493 Google Scholar
- 13 Baker KS, Williams SK, Jarrell BE, Koolpe EA, Levine E. Endothelialization of human collagen surfaces with human adult endothelial cells. Am J Surg 1985; 150: 197–200.
- 14 Barnes CP, Pemble CW, Brand DD, Simpson DG, Bowlin GL. Cross-linking electrospun type II collagen tissue engineering scaffolds with carbodiimide in ethanol. Tissue Eng 2007; 13: 1593–1605.
- 15 Charulatha V, Rajaram A. Influence of different crosslinking treatments on the physical properties of collagen membranes. Biomaterials 2003; 24: 759–767.
- 16 Martelli SM, Moore G, Paes SS, Gandolfo C, Laurindo JB. Influence of plasticizers on the water sorption isotherms and water vapor permeability of chicken feather keratin films. Lwt-Food Sci Technol 2006; 39: 292–301.
- 17 Skotak M, Noriega S, Larsen G, Subramanian A. Electrospun cross-linked gelatin fibers with controlled diameter: The effect of matrix stiffness on proliferative and biosynthetic activity of chondrocytes cultured in vitro. J Biomed Mater Res A 2010; 95: 828–836.
- 18 Zheng WF, Zhang W, Jiang XY. Biomimetic collagen nanofibrous materials for bone tissue engineering. Adv Eng Mater 2010; 12: B451–B466.
- 19 Kew SJ, Gwynne JH, Enea D, Abu-Rub M, Pandit A, Zeugolis D, Brooks RA, Rushton N, Best SM, Cameron RE. Regeneration and repair of tendon and ligament tissue using collagen fibre biomaterials. Acta Biomater 2011; 7: 3237–3247.
- 20 Cegarra J, Gacen J, Caro M. The action of sodium laurylsulfate in the bleaching of wool with hydrogen-peroxide in an acidic medium. J Textile Institu 1983; 74: 351–356.
- 21 Guzman RC, Merrill MR, Richter JR, Hamzi RI, Greengauz-Roberts OK, Van Dyke ME. Mechanical and biological properties of keratose biomaterials. Biomaterials 2011; 32: 8205–8217.
- 22 Weston GJ. The infra-red spectrum of peracetic acid-treated wool. Biochim Biophys Acta 1955; 17: 462–464.
- 23 Metcalfe AD, Ferguson MW. Tissue engineering of replacement skin: The cross-roads of biomaterials, wound healing, embryonic development, stem cells and regeneration. J R Soc Interface 2007; 4: 413–437.
- 24 Lu C, Kang J. Generation of transgenic plants of a potential oilseed crop camelina sativa by agrobacterium-mediated transformation. Plant Cell Rep 2008; 27: 273–278.
- 25 Reddy N, Jin EQ, Chen LH, Jiang X, Yang YQ. Extraction, characterization of components, and potential thermoplastic applications of camelina meal grafted with vinyl monomers. J Agric Food Chem 2012; 60: 4872–4879.
- 26 Vega-Lugo AC, Lim LT. Electrospinning of soy protein isolate nanofibers. J Biobased Mater Bio 2008; 2: 223–230.
- 27 Reddy N, Yang Y. Self-cross-linked gliadin fibers with high strength and water stability for potential medical applications. J Mater Sci Mater Med 2008; 19: 2055–2061.
- 28 Corfield Mc, Robson A. The amino acid composition of wool. Biochem J 1955; 59: 62–68.
- 29 Ber S, Torun Kose G, Hasirci V. Bone tissue engineering on patterned collagen films: An in vitro study. Biomaterials 2005; 26: 1977–1986.
- 30 Rafat M, Matsuura T, Li F, Griffith M. Surface modification of collagen-based artificial cornea for reduced endothelialization. J Biomed Mater Res A 2009; 88: 755–768.
- 31 Choi JS and Yoo HS. Electrospun nanofibers surface-modified with fluorescent proteins. J Bioact Compat Polym 2007; 22: 508–524.
- 32 Jiang QR, Reddy N, Zhang S, Roscioli N, Yang Y. Water-stable electrospun collagen fibers from a non-toxic solvent and crosslinking system. J Biomed Mater Res A 2013; 101: 1237–1247.
- 33 Aluigi A, Vineis C, Tonin C, Tonetti C, Varesano A, Mazzuchetti G. Wool keratin-based nanofibres for active filtration of air and water. J Biobased Mater Bio 2009; 3: 311–319.
- 34 Bader DL, Bowker P. Mechanical characteristics of skin and underlying tissues in vivo. Biomaterials 1983; 4: 305–308.
- 35 Ghosh K, Pan Z, Guan E. Cell adaptation to a physiologically relevant ECM mimic with different viscoelastic properties. Biomaterials 2007; 28: 671–679.
- 36 Lo C-M, Wang H-B, Dembo M, Wang Y-L. Cell movement is guided by the rigidity of the substrate. Biophys J 2000; 79: 144.
- 37 Gualandi C, Soccio M, Govoni M, Valente S, Lotti N, Munari A, Giordano E, Pasquinelli G, Focarete ML. Poly(butylene/diethylene glycol succinate) multiblock copolyester as a candidate biomaterial for soft tissue engineering: Solid-state properties, degradability, and biocompatibility. J Bioact Compat Polym 2012; 27: 244–264.
