Surface immobilization of MEPE peptide onto HA/β-TCP ceramic particles enhances bone regeneration and remodeling†
Bodhraj Acharya
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, Korea
Search for more papers by this authorSo-Young Chun
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, Korea
Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu 700-412, Korea
Search for more papers by this authorShin-Yoon Kim
Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University, Daegu 700-412, Korea
Search for more papers by this authorCheil Moon
Department of Brain Science, Daegu Gyeongbuk Institute of Science & Technology, Daegu 711-873, Korea
Search for more papers by this authorHong-In Shin
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, Korea
Search for more papers by this authorCorresponding Author
Eui Kyun Park
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, Korea
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, KoreaSearch for more papers by this authorBodhraj Acharya
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, Korea
Search for more papers by this authorSo-Young Chun
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, Korea
Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu 700-412, Korea
Search for more papers by this authorShin-Yoon Kim
Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University, Daegu 700-412, Korea
Search for more papers by this authorCheil Moon
Department of Brain Science, Daegu Gyeongbuk Institute of Science & Technology, Daegu 711-873, Korea
Search for more papers by this authorHong-In Shin
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, Korea
Search for more papers by this authorCorresponding Author
Eui Kyun Park
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, Korea
Department of Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu 700-412, KoreaSearch for more papers by this authorHow to cite this article: Acharya B, Chun S-Y, Kim S-Y, Moon C, Shin H-I, Park EK. 2012. Surface immobilization of MEPE peptide onto HA/β-TCP ceramic particles enhances bone regeneration and remodeling. J Biomed Mater Res Part B 2012:100B:841–849.
Abstract
Calcium phosphate ceramics have been widely used as scaffolds for bone regeneration. Here, to improve the osteogenic potential of hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) and to apply the bioactive peptide in situ, matrix extracellular phosphoglycoprotein (MEPE) peptide, which has been shown to stimulate osteoblast differentiation, was covalently and directionally immobilized on HA/β-TCP particles. The free-hydroxyl groups on the surface of the HA/β-TCP particles were sequentially conjugated with APTES, PEG-(SS)2, and the synthetic MEPE peptide. Using FTIR and XPS, immobilization of the MEPE peptide on the HA/β-TCP was confirmed. Implantation of the MEPE peptide-immobilized HA/β-TCP into calvarial defect and subsequent analyses using a micro CT and histology showed significant bone regeneration and increased bone area (9.89-fold) as compared to that of unmodified HA/β-TCP. Moreover, tartrate-resistant acid phosphatase-positive osteoclasts were observed in regenerated bone by the MEPE peptide-immobilized HA/β-TCP, indicating that the bones newly formed by the MEPE peptide-immobilized HA/β-TCP are actively remodeled by osteoclasts. Therefore, our data demonstrate that MEPE peptide immobilization onto the HA/β-TCP surface stimulates bone regeneration associated with physiological bone remodeling. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Supporting Information
Additional Supporting Information may be found in the online version of this article.
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| JBM_32648_sm_SuppFig1.tif1.3 MB | Supporting Information Figure 1 |
| JBM_32648_sm_SuppFig2.tif1.8 MB | Supporting Information Figure 2 |
| JBM_32648_sm_SuppFig3.tif1.4 MB | Supporting Information Figure 3 |
| JBM_32648_sm_SuppFig4.tif1.3 MB | Supporting Information Figure 4 |
| JBM_32648_sm_SuppFig5.tif17.6 MB | Supporting Information Figure 5 |
| JBM_32648_sm_SuppFig6.tif8 MB | Supporting Information Figure 6 |
| JBM_32648_sm_SuppFig7.tif6.5 MB | Supporting Information Figure 7 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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