Micro-CT-based bone ceramic scaffolding and its performance after seeding with mesenchymal stem cells for repair of load-bearing bone defect in canine femoral head
Jiang Peng
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
These authors contributed equally to this work.
Search for more papers by this authorCunyi Wen
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
These authors contributed equally to this work.
Search for more papers by this authorAiyuan Wang
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
These authors contributed equally to this work.
Search for more papers by this authorYu Wang
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorWenjing Xu
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorBin Zhao
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorLi Zhang
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorCorresponding Author
Shibi Lu
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Shibi Lu, Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Ling Qin, Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
Search for more papers by this authorCorresponding Author
Ling Qin
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
Shibi Lu, Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Ling Qin, Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
Search for more papers by this authorQuanyi Guo
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorLimin Dong
Institute of Nuclear Energy Technology, Tsinghua University, Beijing, 100084, China
Search for more papers by this authorJiemo Tian
Institute of Nuclear Energy Technology, Tsinghua University, Beijing, 100084, China
Search for more papers by this authorJiang Peng
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
These authors contributed equally to this work.
Search for more papers by this authorCunyi Wen
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
These authors contributed equally to this work.
Search for more papers by this authorAiyuan Wang
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
These authors contributed equally to this work.
Search for more papers by this authorYu Wang
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorWenjing Xu
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorBin Zhao
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorLi Zhang
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorCorresponding Author
Shibi Lu
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Shibi Lu, Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Ling Qin, Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
Search for more papers by this authorCorresponding Author
Ling Qin
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
Shibi Lu, Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Ling Qin, Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China
Search for more papers by this authorQuanyi Guo
Orthopaedic Research Institute of Chinese People Liberation Army, The Chinese PLA General Hospital, Beijing, China
Search for more papers by this authorLimin Dong
Institute of Nuclear Energy Technology, Tsinghua University, Beijing, 100084, China
Search for more papers by this authorJiemo Tian
Institute of Nuclear Energy Technology, Tsinghua University, Beijing, 100084, China
Search for more papers by this authorAbstract
Osteonecrosis of the femoral head is a debilitating and painful orthopedic condition characterized by joint collapse. Salvage of the femoral head is highly desirable to preserve the contour and mechanical properties and prevent joint collapse. This study aimed to develop a new tissue-engineering approach for treatment of large bone defect in femoral head, that is, after osteonecrosis. The biphasic calcium phosphate (BCP) ceramic scaffolds were fabricated by a 3D gel-lamination technique based on micro-computed tomography (micro-CT) images of the cancellous bone microarchitecture of femoral heads. After seeding with autologous bone marrow-derived mesenchymal stem cells (BMSCs) in vitro, the cell-scaffold composite was implanted into a bone defect surgically induced in canine femoral head via trapdoor procedure, which was a common procedure for treatment of osteonecrosis. A total of 24 adult dogs were randomly divided into three groups (n = 8 each) for implantation of the BCP scaffold with or without with BMSCs, and also the autologous bone chips for comparisons. All animals were sacrificed at 30 weeks postoperatively and processed for radiological and histological evaluations. The contour of the femoral head was well preserved with implantation of BCP scaffolds with or without BMSCs, whereas joint collapse was found after treatment with autologous bone chips. The osteointegration and new bone formation was significantly greater with BCP scaffold implantation with than without BMSC seeding and showed greater strength and compressive modulus in the repair site. Micro-CT-based bone ceramic scaffolds seeding with BMSC might be a promising way to repair bone defects in the femoral head. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
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