Validation of the CDC biofilm reactor as a dynamic model for assessment of encrustation formation on urological device materials
Brendan F. Gilmore,
Turlough M. Hamill,
David S. Jones,
Sean P. Gorman,
Corresponding Author
Brendan F. Gilmore
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UKSearch for more papers by this authorTurlough M. Hamill
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK
Search for more papers by this authorDavid S. Jones
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK
Search for more papers by this authorSean P. Gorman
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK
Search for more papers by this authorBrendan F. Gilmore,
Turlough M. Hamill,
David S. Jones,
Sean P. Gorman,
Corresponding Author
Brendan F. Gilmore
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UKSearch for more papers by this authorTurlough M. Hamill
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK
Search for more papers by this authorDavid S. Jones
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK
Search for more papers by this authorSean P. Gorman
School of Pharmacy, Queens University Belfast, Medical Biology Centre, Belfast, BT9 7BL, UK
Search for more papers by this authorAbstract
Contemporary medical science is reliant upon the rational selection and utilization of devices, and therefore, an increasing need has developed for in vitro systems aimed at replicating the conditions to which urological devices will be subjected to during their use in vivo. We report the development and validation of a novel continuous flow encrustation model based on the commercially available CDC biofilm reactor. Proteus mirabilis-induced encrustation formation on test biomaterial sections under varying experimental parameters was analyzed by X-ray diffraction, infrared- and Raman spectroscopy and by scanning electron microscopy. The model system produced encrusted deposits similar to those observed in archived clinical samples. Results obtained for the system are highly reproducible with encrustation being rapidly deposited on test biomaterial sections. This model will have utility in the rapid screening of encrustation behavior of biomaterials for use in urological applications. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
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