Evaluation of encapsulated Newcastle disease virus liposomes using various phospholipids administered to improve chicken humoral immunity
Li-Ping Tseng
Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan
Search for more papers by this authorChwei-Jang Chiou
Graduate Institute of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
Search for more papers by this authorMing-Chung Deng
Department of Hog Cholera, Animal Health Research Institute, Council of Agriculture, Taipei, Taiwan
Search for more papers by this authorMei-Hsiu Lin
Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
Search for more papers by this authorRyh-Nan Pan
Department of Disaster Management, Taiwan Police College, Taipei, Taiwan
Search for more papers by this authorCorresponding Author
Yi-You Huang
Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan
Yi-You Huang and Der-Zen Liu contributed equally to this work.
Yi-You Huang, Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan
Der-Zen Liu, Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
Search for more papers by this authorCorresponding Author
Der-Zen Liu
Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
Yi-You Huang and Der-Zen Liu contributed equally to this work.
Yi-You Huang, Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan
Der-Zen Liu, Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
Search for more papers by this authorLi-Ping Tseng
Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan
Search for more papers by this authorChwei-Jang Chiou
Graduate Institute of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
Search for more papers by this authorMing-Chung Deng
Department of Hog Cholera, Animal Health Research Institute, Council of Agriculture, Taipei, Taiwan
Search for more papers by this authorMei-Hsiu Lin
Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
Search for more papers by this authorRyh-Nan Pan
Department of Disaster Management, Taiwan Police College, Taipei, Taiwan
Search for more papers by this authorCorresponding Author
Yi-You Huang
Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan
Yi-You Huang and Der-Zen Liu contributed equally to this work.
Yi-You Huang, Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan
Der-Zen Liu, Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
Search for more papers by this authorCorresponding Author
Der-Zen Liu
Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
Yi-You Huang and Der-Zen Liu contributed equally to this work.
Yi-You Huang, Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei, Taiwan
Der-Zen Liu, Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
Search for more papers by this authorAbstract
We propose the adjuvant effects of phospholipid liposome compositions using intranasal inoculation of a liposomal-Newcastle disease virus (NDV) vaccine in chickens. The immunogenicity of three liposome formulations was determined in chickens using the hemagglutination-inhibition (HI) test, nasal secretory immunoglobulin A and serum immunoglobulin A (IgG) antibody titers using the enzyme-linked immunosorbent assay. The immune response against NDV antigens was determined after immunization with neutral charged liposomes composed of egg phosphatidylcholine (EPC) (60 μmol), cholesterol (Chol) (15 μmol), and EPC-liposomes (EPC-Lip), which elicited strong systemic (serum) and local (nasal) humoral responses. However, the intranasal administration with cationic charged liposomes composed of EPC (30 μmol), stearylamine (SA) (15 μmol), Chol (15 μmol), and SA-liposomes (SA-Lip) induced poor humoral immune responses. Only the vaccine formulated with anionic charged liposomes composed of EPC (30 μmol), dipalmitoylphosphatidylserine (15 μmol), Chol (15 μmol), and phosphatidylserine-liposomes (PS-Lip) elicited the highest titers of HI antibodies. These are the first results to suggest that antigen delivery using EPC-Lip is very useful in enhancing antibody production at the mucosal site and in serum. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
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