Bioreactor cultivation condition for engineered bone tissue: Effect of various bioreactor designs on extra cellular matrix synthesis
Hanieh Nokhbatolfoghahaei
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMahboubeh Bohlouli
Student Research Committee, Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorZahrasadat Paknejad
Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMaryam R. Rad
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorLeila M. Amirabad
School of Dentistry, Marquette University, Milwaukee, Wisconsin, USA
Search for more papers by this authorNasim Salehi-Nik
Department of Biomechanical Engineering, Faulty of Engineering Technology, University of Twente, Enschede, The Netherlands
Search for more papers by this authorMohammad M. Khani
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorShayan Shahriari
Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
Search for more papers by this authorNasser Nadjmi
The Team for Cleft and Craniofacial Anomalies, Oral and Maxillofacial Surgery, University of Antwerp, Antwerp, Belgium
Search for more papers by this authorAdel Ebrahimpour
Department of Orthopedics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorCorresponding Author
Arash Khojasteh
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Correspondence
Arash Khojasteh, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Email: [email protected]
Search for more papers by this authorHanieh Nokhbatolfoghahaei
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMahboubeh Bohlouli
Student Research Committee, Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorZahrasadat Paknejad
Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMaryam R. Rad
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorLeila M. Amirabad
School of Dentistry, Marquette University, Milwaukee, Wisconsin, USA
Search for more papers by this authorNasim Salehi-Nik
Department of Biomechanical Engineering, Faulty of Engineering Technology, University of Twente, Enschede, The Netherlands
Search for more papers by this authorMohammad M. Khani
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorShayan Shahriari
Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
Search for more papers by this authorNasser Nadjmi
The Team for Cleft and Craniofacial Anomalies, Oral and Maxillofacial Surgery, University of Antwerp, Antwerp, Belgium
Search for more papers by this authorAdel Ebrahimpour
Department of Orthopedics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorCorresponding Author
Arash Khojasteh
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Correspondence
Arash Khojasteh, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Email: [email protected]
Search for more papers by this authorFunding information: Iran Ministry of Health and Medical Education, Grant/Award Number: 700/1702
Abstract
Dynamic-based systems are bio-designed in order to mimic the micro-environments of the bone tissue. There is limited direct comparison between perfusion and perfusion-rotation forces in designing a bioreactor. Hence, in current study, we aimed to compare given bioreactors for bone regeneration. Two types of bioreactors including rotating & perfusion and perfusion bioreactors were designed. Mesenchymal stem cells derived from buccal fat pad were loaded on a gelatin/β-Tricalcium phosphate scaffold. Cell-scaffold constructs were subjected to different treatment condition and place in either of the bioreactors. Effect of different dynamic conditions on cellular behavior including cell proliferation, cell adhesion, and osteogenic differentiation were assessed. Osteogenic assessment of scaffolds after 24 days revealed that rotating & perfusion bioreactor led to significantly higher expression of OCN and RUNX2 genes and also greater amount of ALP and collagen I protein production compared to static groups and perfusion bioreactor. Observation of cellular sheets which filled the scaffold porosities in SEM images, approved the better cell responses to rotating & perfusion forces of the bioreactor. The outcomes demonstrated that rotating & perfusion bioreactor action on bone regeneration is much preferable than perfusion bioreactor. Therefore, it seems that exertion of multi-stimuli is more effective for bone engineering.
CONFLICT OF INTEREST
The authors declare no potential conflict of interest.
Supporting Information
| Filename | Description |
|---|---|
| jbma36932-sup-0001-supinfo.docxWord 2007 document , 17.5 KB | Appendix S1. Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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