Dynamic cell patterning of photoresponsive hyaluronic acid hydrogels
Catherine A. Goubko
Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, Ontario, K1N 6N5 Canada
Search for more papers by this authorAjoy Basak
Ottawa Hospital Research Institute, Loeb Building, Chronic Disease Program, The Ottawa Hospital, University of Ottawa, 725 Parkdale Avenue, Ottawa, Ontario, K1Y 4Y9 Canada
Search for more papers by this authorSwapan Majumdar
Ottawa Hospital Research Institute, Loeb Building, Chronic Disease Program, The Ottawa Hospital, University of Ottawa, 725 Parkdale Avenue, Ottawa, Ontario, K1Y 4Y9 Canada
Search for more papers by this authorCorresponding Author
Xudong Cao
Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, Ontario, K1N 6N5 Canada
Ottawa-Carleton Institute for Biomedical Engineering, University of Ottawa, Ottawa, Ontario, K1N 6N5 Canada
Correspondence to: X. Cao; e-mail: [email protected]Search for more papers by this authorCatherine A. Goubko
Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, Ontario, K1N 6N5 Canada
Search for more papers by this authorAjoy Basak
Ottawa Hospital Research Institute, Loeb Building, Chronic Disease Program, The Ottawa Hospital, University of Ottawa, 725 Parkdale Avenue, Ottawa, Ontario, K1Y 4Y9 Canada
Search for more papers by this authorSwapan Majumdar
Ottawa Hospital Research Institute, Loeb Building, Chronic Disease Program, The Ottawa Hospital, University of Ottawa, 725 Parkdale Avenue, Ottawa, Ontario, K1Y 4Y9 Canada
Search for more papers by this authorCorresponding Author
Xudong Cao
Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, Ontario, K1N 6N5 Canada
Ottawa-Carleton Institute for Biomedical Engineering, University of Ottawa, Ottawa, Ontario, K1N 6N5 Canada
Correspondence to: X. Cao; e-mail: [email protected]Search for more papers by this authorAbstract
Techniques to pattern cells on biocompatible hydrogels allow for the creation of highly controlled cell microenvironments within materials that mimic the physicochemical properties of native tissues. Such technology has the potential to further enhance our knowledge of cell biology and to play a role in the development of novel tissue engineering devices. Light is an ideal stimulus to catalyze pattern formation since it can be controlled spatially as well as temporally. Herein, we have developed and enhanced a hydrogel cell patterning strategy. It is based on photoactive caged RGDS peptides incorporated into a hyaluronic acid (HA) hydrogel, which can be subsequently activated with near-UV light to create cell-adhesive regions within an otherwise non-adhesive hydrogel. With this strategy, we have been able to pattern multiple cell populations—either in contact with one another or held apart—on an underlying chemically patterned HA hydrogel. Furthermore, the hydrogel cell pattern could be altered with time, even 2 weeks after initial seeding, to create additional adhesive regions to regulate the direction of cell growth and migration. These dynamic hydrogel cell patterns, created with a standard fluorescence microscope, were shown to be robust and lasted at least 3 weeks in vitro. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 381–391, 2014.
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