Journal of Biomedical Materials Research Part A

Synthesis, characterization, and biocompatibility of a novel biomimetic material based on MGF-Ct24E modified poly(D, L-lactic acid)^†

Yuxiao Li

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Bingbing Zhang,

Bingbing Zhang

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Changshun Ruan,

Changshun Ruan

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Pinpin Wang,

Pinpin Wang

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Jiaoxia Sun,

Jiaoxia Sun

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Jun Pan,

Jun Pan

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Yuanliang Wang,

Corresponding Author

Yuanliang Wang

[email protected]

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, ChinaSearch for more papers by this author

Yuxiao Li,

Yuxiao Li

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Bingbing Zhang,

Bingbing Zhang

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Changshun Ruan,

Changshun Ruan

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Pinpin Wang,

Pinpin Wang

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Jiaoxia Sun,

Jiaoxia Sun

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Jun Pan,

Jun Pan

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Search for more papers by this author

Yuanliang Wang,

Corresponding Author

Yuanliang Wang

[email protected]

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China

Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, ChinaSearch for more papers by this author

First published: 31 August 2012

https://doi.org/10.1002/jbm.a.34276

Citations: 10

^†

How to cite this article: Li Y, Zhang B, Ruan C, Wang P, Sun J, Pan J, Wang Y. 2012. Synthesis, characterization, and biocompatibility of a novel biomimetic material based on MGF-Ct24E modified poly(D, L-lactic acid). J Biomed Mater Res Part A 2012:100A:3496–3502.

Share a link

Email
Wechat
Bluesky

Abstract

Mechano-growth factor (MGF) is an alternative splicing variant of Insulin-like growth factor I. MGF and its 24 amino acid peptide analog corresponding to the unique C-terminal E-domain (MGF-Ct24E) are the positive regulator for tissue regenesis in bone. A novel biomimetic poly(D, L-lactic acid) (PDLLA) modification was designed and synthesized based on MGF-Ct24E grafted maleic anhydride modified PDLLA (MPLA). MGF-Ct24Es were grafted into the side chain of MPLA via a stable covalent amide bond using 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as the condensing agent to produce biomimetic MPLA materials (MGF-Ct24E-MPLA). Fourier transform infrared spectrometry, amino acid analyzer, and elementary analysis were used to characterize the MGF-Ct24E-MPLA. The hydrophilicity of MGF-Ct24E-MPLA was evaluated by means of the water-uptake ratios and static water contact angle. Data revealed that the grafting efficiency of MGF-Ct24E was about 29.9%. MGF-Ct24E-MPLA had better hydrophilicity than PDLLA and MPLA. The osteoblasts behavior of proliferation, differentiation, and mineralization on PDLLA, MPLA, and MGF-Ct24E-MPLA films was investigated and the results indicated that the introduction of MGF-Ct24E could improve osteoblasts proliferation, mineralization, and delay differentiation. The MGF-Ct24E modified MPLA with higher bioactivity may have potential application for bone tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3496–3502, 2012.

REFERENCES

1 Bostman O, Pihlajamaki H. Clinical biocompatibility of biodegradable orthopaedic implants for internal fixation: A review. Biomaterials 2000; 21: 2615–2621.
10.1016/S0142-9612(00)00129-0
CAS PubMed Web of Science® Google Scholar
2 Luo YF, Wang YL, Niu XF, Pan J, Shi LP. Synthesis and characterization of a novel biomaterial: Maleic anhydride-modified poly(D, L-lactic acid). Chin Chem Lett 2004; 15: 521–524.
CAS Web of Science® Google Scholar
3 Niu X, Luo Y, Li Y, Fu C, Chen J, Wang Y. Design of bioinspired polymeric materials based on poly(D, L-lactic acid) modifications towards improving its cytocompatibility. J Biomed Mater Res A 2008; 84: 908–916.
10.1002/jbm.a.31360
CAS PubMed Web of Science® Google Scholar
4 Cui W, Li X, Xie, Chen J, Zou J, Zhou S, Weng J. Controllable growth of hydroxyapatite on electrospun poly(dl-lactide) fibers grafted with chitosan as potential tissue engineering scaffolds. Polymer 2010; 51: 2320–2328.
10.1016/j.polymer.2010.03.037
CAS Web of Science® Google Scholar
5 Elisseeff J, Anseth K, Langer R, Hrkach J. Synthesis and characterization of photo-cross-linked polymers based on poly(L-lactic acid-co-L-aspartic acid). Macromolecules 1997; 30: 2182–2184.
10.1021/ma961411w
CAS Web of Science® Google Scholar
6 Wang Z, Zhao H, Wang Q, Ye R, David E. Synthesis of poly (D, L-lactic acid) modified by cholic acid via direct melt copolycondensation and its characterization. J Appl Polym Sci 2010; 117: 1405–1415.
10.1002/app.31937
CAS Web of Science® Google Scholar
7 Arvanitoyannis I, Nakayama A, Kawasaki N, Yamamoto N. Novel star-shaped polylactide with glycerol using stannous octoate or tetraphenyl tin as catalyst: 1. Synthesis, characterization and study of their biodegradability. Polymer 1995; 36: 2947–2956.
10.1016/0032-3861(95)94344-S
CAS Web of Science® Google Scholar
8 Cannizzaro SM, Padera RF, Langer R, Rogers RA, Black FE, Davies MC, Tendler SJB, Shakesheff KM. A novel biotinylated degradable polymer for cell-interactive applications. Biotechnol Bioeng 1998; 58: 529–535.
10.1002/(SICI)1097-0290(19980605)58:5<529::AID-BIT9>3.0.CO;2-F
CAS PubMed Web of Science® Google Scholar
9 Li J, Zhai Y, Zhang B, Deng L. Methoxy poly (ethylene glycol)-block-poly (D, L-lactic acid) copolymer nanoparticles as carriers for transdermal drug delivery. Polym Int 2008; 57: 268–274.
10.1002/pi.2339
CAS Web of Science® Google Scholar
10 Ruan C, Wang Y, Zhang M, Luo Y, Fu C, Huang M, Sun J, Hu C. Design, synthesis and characterization of novel biodegradable shape memory polymers based on poly(D, L-lactic acid) diol, hexamethylene diisocyanate and piperazine. Polym Int 2011; 61: 524–530.
10.1002/pi.3197
CAS Web of Science® Google Scholar
11 Carlisle ES, Mariappan MR, Nelson KD, Thomes BE, Timmons RB, Constantinescu A, Eberhart RC, Bankey PE. Enhancing hepatocyte adhesion by pulsed plasma deposition and polyethylene glycol coupling. Tissue Eng 2000; 6: 45–52.
10.1089/107632700320883
CAS PubMed Web of Science® Google Scholar
12 Sodergard A. Modification of polylactide. Recent Res Dev Polym Sci 1998; 2: 263–275.
CAS Google Scholar
13 Wan Y, Fang Y, Wu H, Cao X. Porous polylactide/chitosan scaffolds for tissue engineering. J Biomed Mater Res A 2007; 80: 776–789.
10.1002/jbm.a.31025
CAS PubMed Web of Science® Google Scholar
14 Niu X, Feng Q, Wang M, Guo X, Zheng Q. Porous nano-HA/collagen/PLLA scaffold containing chitosan microspheres for controlled delivery of synthetic peptide derived from BMP-2. J Control Release 2009; 134: 111–117.
10.1016/j.jconrel.2008.11.020
CAS PubMed Web of Science® Google Scholar
15 Niu X, Wang Y, Luo Y, Pan J, Shang J, Guo L. Synthesis of the biomimetic polymer: aliphatic diamine and rgds modified poly(D,L-lactic acid). Chin Chem Lett 2005; 16: 1035–1038.
CAS Web of Science® Google Scholar
16 Luo Y, Wang S, Niu X, Wang Y. Synthesis and characterization of collagen type I-modified poly(D,L-lactic acid) biomimetic material. J Funct Mater 2008; 1: 123–126.
Web of Science® Google Scholar
17 Dai Z, Wu F, Yeung EW, Li Y. IGF-IEc expression, regulation and biological function in different tissues. Growth Horm IGF Res 2010; 20: 275–281.
10.1016/j.ghir.2010.03.005
CAS PubMed Web of Science® Google Scholar
18 Goldspink G. Impairment of IGF-I gene splicing and MGF expression associated with muscle wasting. Int J Biochem Cell Biol 2006; 38: 481–489.
10.1016/j.biocel.2005.10.001
CAS PubMed Web of Science® Google Scholar
19 Yang S.Y, Goldspink G. Different roles of the IGF-I Ec peptide (MGF) and mature IGF-I in myoblast proliferation and differentiation. FEBS Lett 2002; 522: 156–160.
10.1016/S0014-5793(02)02918-6
CAS PubMed Web of Science® Google Scholar
20 Zhang B, Jiang P, Xian C, Li Y, Li D, Tang L, Wang Y. Expression of mechano-growth factor in Escherichia coli and activity analysis. Chin J Biotechnol 2008; 24: 1180–1185.
CAS PubMed Web of Science® Google Scholar
21 Robey PG, Ternube JD. Human bone cells in vitro. Calcified Tissue Int 1985; 37: 453–460.
10.1007/BF02557826
CAS PubMed Web of Science® Google Scholar
22 Tang LL, Wang YL, Gu L, Su AH, Cai SX. The physiological response of osteoblasts to a gradeently increased stretching. Acta Biophys Sin 2003; 19: 88–91.
Google Scholar
23 Gregory CA, Gunn WG, Peister A, Prockop DJ. An Alizarin red-based assay of mineralization by adherent cells in culture: Comparison with cetylpyridinium chloride extraction. Anal Biochem 2004; 329: 77–84.
10.1016/j.ab.2004.02.002
CAS PubMed Web of Science® Google Scholar
24 Tullberg-Reinert H, Jundt G. In situ measurement of collagen synthesis by human bone cells with a Sirius Red-based colorimetric microassay: Effects of transforming growth factor β2 and ascorbic acid 2-phosphate. Histochem Cell Biol 1999; 112: 271–276.
10.1007/s004180050447
CAS PubMed Web of Science® Google Scholar
25 Massia SP, Hubbell JA. An RGD spacing of 440 nm is sufficient for integrin alpha V beta 3-mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation. J Cell Biol 1991; 114: 1089–1100.
10.1083/jcb.114.5.1089
CAS PubMed Web of Science® Google Scholar
26 Deng M, Zhang B, Wang K, Liu F, Xiao H, Zhao J, Liu P, Li Y, Lin F, Wang Y. Mechano growth factor E peptide promotes osteoblasts proliferation and bone-defect healing in rabbits. Int Orthop 2011; 35: 1099–1106.
10.1007/s00264-010-1141-2
PubMed Web of Science® Google Scholar

Citing Literature

Volume100A, Issue12

December 2012

Pages 3496-3502

Synthesis, characterization, and biocompatibility of a novel biomimetic material based on MGF-Ct24E modified poly(D, L-lactic acid)^†

Abstract

REFERENCES

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Synthesis, characterization, and biocompatibility of a novel biomimetic material based on MGF-Ct24E modified poly(D, L-lactic acid)†

Abstract

REFERENCES

Citing Literature

References

Related

Information

Synthesis, characterization, and biocompatibility of a novel biomimetic material based on MGF-Ct24E modified poly(D, L-lactic acid)^†