Volume 88A, Issue 3 pp. 823-831

Stereo imaging and cytocompatibility of a model dental implant surface formed by direct laser fabrication

Carlo Mangano

Carlo Mangano

Dental School, Insubria University, Varese, Italy

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Mario Raspanti

Mario Raspanti

Department of Human Morphology, Insubria University, Varese, Italy

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Tonino Traini

Tonino Traini

Dental School, University of Chieti-Pescara, Chieti, Italy

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Adriano Piattelli

Adriano Piattelli

Dental School, University of Chieti-Pescara, Chieti, Italy

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Rachel Sammons

Corresponding Author

Rachel Sammons

Dental School, University of Birmingham, Birmingham, United Kingdom

Dental School, University of Birmingham, Birmingham, United KingdomSearch for more papers by this author
First published: 15 May 2008
Citations: 52

Abstract

Direct laser fabrication (DLF) allows solids with complex geometry to be produced by sintering metal powder particles in a focused laser beam. In this study, 10 Ti6Al4V alloy model dental root implants were obtained by DLF, and surface characterization was carried out using stereo scanning electron microscopy to produce 3D reconstructions. The surfaces were extremely irregular, with ∼100 μm deep, narrow intercommunicating crevices, shallow depressions and deep, rounded pits of widely variable shape and size, showing ample scope for interlocking with the host bone. Roughness parameters were as follows: Rt, 360.8 μm; Rz, 358.4 μm; Ra, 67.4 μm; and Rq, 78.0 μm. Disc specimens produced by DLF with an identically prepared surface were used for biocompatibility studies with rat calvarial osteoblasts: After 9 days, cells had attached and spread on the DLF surface, spanning across the crevices, and voids. Cell density was similar to that on a commercial rough microtextured surface but lower than on commercial smooth machined and smooth-textured grit-blasted, acid-etched surfaces. Human fibrin clot extension on the DLF surface was slightly improved by inorganic acid etching to increase the microroughness. With further refinements, DLF could be an economical means of manufacturing implants from titanium alloys. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009

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