Nano hydroxyapatite structures influence early bone formation
Corresponding Author
Luiz Meirelles
Department of Prosthetic Dentistry/Dental Material Science, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
Department of Biomaterials, Sahlgrenska Academy at Göteborg University, Goteborg, Sweden
Department of Prosthetic Dentistry/Dental Material Science, Sahlgrenska Academy at Göteborg University, Göteborg, SwedenSearch for more papers by this authorAnna Arvidsson
Department of Biomaterials, Sahlgrenska Academy at Göteborg University, Goteborg, Sweden
Search for more papers by this authorMartin Andersson
Department of Applied Surface Chemistry, Chalmers University of Technology, Göteborg, Sweden
Search for more papers by this authorPer Kjellin
Department of Applied Surface Chemistry, Chalmers University of Technology, Göteborg, Sweden
Search for more papers by this authorTomas Albrektsson
Department of Biomaterials, Sahlgrenska Academy at Göteborg University, Goteborg, Sweden
Search for more papers by this authorAnn Wennerberg
Department of Prosthetic Dentistry/Dental Material Science, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
Department of Biomaterials, Sahlgrenska Academy at Göteborg University, Goteborg, Sweden
Search for more papers by this authorCorresponding Author
Luiz Meirelles
Department of Prosthetic Dentistry/Dental Material Science, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
Department of Biomaterials, Sahlgrenska Academy at Göteborg University, Goteborg, Sweden
Department of Prosthetic Dentistry/Dental Material Science, Sahlgrenska Academy at Göteborg University, Göteborg, SwedenSearch for more papers by this authorAnna Arvidsson
Department of Biomaterials, Sahlgrenska Academy at Göteborg University, Goteborg, Sweden
Search for more papers by this authorMartin Andersson
Department of Applied Surface Chemistry, Chalmers University of Technology, Göteborg, Sweden
Search for more papers by this authorPer Kjellin
Department of Applied Surface Chemistry, Chalmers University of Technology, Göteborg, Sweden
Search for more papers by this authorTomas Albrektsson
Department of Biomaterials, Sahlgrenska Academy at Göteborg University, Goteborg, Sweden
Search for more papers by this authorAnn Wennerberg
Department of Prosthetic Dentistry/Dental Material Science, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
Department of Biomaterials, Sahlgrenska Academy at Göteborg University, Goteborg, Sweden
Search for more papers by this authorAbstract
In a study model that aims to evaluate the effect of nanotopography on bone formation, micrometer structures known to alter bone formation, should be removed. Electropolished titanium implants were prepared to obtain a surface topography in the absence of micro structures, thereafter the implants were divided in two groups. The test group was modified with nanosize hydroxyapatite particles; the other group was left uncoated and served as control for the experiment. Topographical evaluation demonstrated increased nanoroughness parameters for the nano-HA implant and higher surface porosity compared to the control implant. The detected features had increased size and diameter equivalent to the nano-HA crystals present in the solution and the relative frequency of the feature size and diameter was very similar. Furthermore, feature density per μm2 showed a decrease of 13.5% on the nano-HA implant. Chemical characterization revealed calcium and phosphorous ions on the modified implants, whereas the control implants consisted of pure titanium oxide. Histological evaluation demonstrated significantly increased bone formation to the coated (p < 0.05) compared to uncoated implants after 4 weeks of healing. These findings indicate for the first time that early bone formation is dependent on the nanosize hydroxyapatite features, but we are unaware if we see an isolated effect of the chemistry or of the nanotopography or a combination of both. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
References
- 1 Curtis A,Wilkinson C. Topographical control of cells. Biomaterials 1997; 18: 1573–1583.
- 2 Boyan BD,Hummert TW,Dean DD,Schwartz Z. Role of material surfaces in regulating bone and cartilage cell response. Biomaterials 1996; 17: 137–146.
- 3 Chehroudi B,Gould TR,Brunette DM. Effects of a grooved titanium-coated implant surface on epithelial cell behavior in vitro and in vivo. J Biomed Mater Res 1989; 23: 1067–1085.
- 4 Wennerberg A. Biomaterials Science/Handicap Research. Göteborg: Göteborg University; 1996.
- 5 Gottlander M,Johansson CB,Wennerberg A,Albrektsson T,Radin S,Ducheyne P. Bone tissue reactions to an electrophoretically applied calcium phosphate coating. Biomaterials 1997; 18: 551–557.
- 6 Walivaara B,Aronsson BO,Rodahl M,Lausmaa J,Tengvall P. Titanium with different oxides: In vitro studies of protein adsorption and contact activation. Biomaterials 1994; 15: 827–834.
- 7 Ellingsen JE. A study on the mechanism of protein adsorption to TiO2. Biomaterials 1991; 12: 593–596.
- 8
Hay ED.
Cell Biology of Extracellular Matrix.
New York:
Plenum;
1991.
10.1007/978-1-4615-3770-0_13 Google Scholar
- 9 Wojciak-Stothard B,Curtis A,Monaghan W,MacDonald K,Wilkinson C. Guidance and activation of murine macrophages by nanometric scale topography. Exp Cell Res 1996; 223: 426–435.
- 10 Shirato I,Tomino Y,Koide H,Sakai T. Fine structure of the glomerular basement membrane of the rat kidney visualized by high-resolution scanning electron microscopy. Cell Tissue Res 1991; 266: 1–10.
- 11 Abrams GA,Goodman SL,Nealey PF,Franco M,Murphy CJ. Nanoscale topography of the basement membrane underlying the corneal epithelium of the rhesus macaque. Cell Tissue Res 2000; 299: 39–46.
- 12 Curtis A,Wilkinson C. New depths in cell behaviour: Reactions of cells to nanotopography. Biochem Soc Symp 1999; 65: 15–26.
- 13 Flemming RG,Murphy CJ,Abrams GA,Goodman SL,Nealey PF. Effects of synthetic micro- and nano-structured surfaces on cell behavior. Biomaterials 1999; 20: 573–588.
- 14 Bloebaum RD,Beeks D,Dorr LD,Savory CG,DuPont JA,Hofmann AA. Complications with hydroxyapatite particulate separation in total hip arthroplasty. Clin Orthop Relat Res 1994: 19–26.
- 15 Andersson AS,Backhed F,von Euler A,Richter-Dahlfors A,Sutherland D,Kasemo B. Nanoscale features influence epithelial cell morphology and cytokine production. Biomaterials 2003; 24: 3427–3436.
- 16 Teixeira AI,Abrams GA,Bertics PJ,Murphy CJ,Nealey PF. Epithelial contact guidance on well-defined micro- and nanostructured substrates. J Cell Sci 2003; 116: 1881–1892.
- 17 Webster TJ,Ergun C,Doremus RH,Siegel RW,Bizios R. Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics. J Biomed Mater Res 2000; 51: 475–483.
- 18 Zhu X,Eibl O,Scheideler L,Geis-Gerstorfer J. Characterization of nano hydroxyapatite/collagen surfaces and cellular behaviors. J Biomed Mater Res A 2006; 79: 114–127.
- 19 Webster TJ,Siegel RW,Bizios R. Osteoblast adhesion on nanophase ceramics. Biomaterials 1999; 20: 1221–1227.
- 20 Zhu XL,Eibl O,Berthold C,Scheideler L,Geis-Gerstrofer J. Structural characterization of nanocrystalline hydroxyapatite and adhesion of pre-osteoblast cells. Nanotechnology 2006; 17: 2711–2721.
- 21 Webster TJ,Ergun C,Doremus RH,Siegel RW,Bizios R. Enhanced functions of osteoblasts on nanophase ceramics. Biomaterials 2000; 21: 1803–1810.
- 22 Albrektsson T,Albrektsson B. Microcirculation in grafted bone. A chamber technique for vital microscopy of rabbit bone transplants. Acta Orthop Scand 1978; 49: 1–7.
- 23 Albrektsson T. Repair of bone grafts. A vital microscopic and histological investigation in the rabbit. Scand J Plast Reconstr Surg 1980; 14: 1–12.
- 24 Carlsson L,Rostlund T,Albrektsson B,Albrektsson T. Implant fixation improved by close fit. Cylindrical implant-bone interface studied in rabbits. Acta Orthop Scand 1988; 59: 272–275.
- 25 Meirelles L,Arvidsson A,Albrektsson T,Wennerberg A. Increased bone formation to nano rough unstable implant. Clin Oral Implants Res 2007; 18: 326–332.
- 26 Piotrowski O,Madore C,Landolt D. The mechanism of electropolishing of titanium in methanol-sulfuric acid electrolytes. J Electrochem Soc 1998; 145: 2362–2369.
- 27 Kjellin P,Andersson M. SE-0401524-4, assignee. Synthetic nano-sized crystalline calcium phosphate and method of production patent SE527610. 2006. 25 April 2006.
- 28 Stout K-J SP,Dong WP,Mainsah E,Luo N,Mathia T,Zahouani H. The development of methods for characterization of roughness in three dimensions. EUR 15178 EN of Commission of the European Communities, University of Birmingham, Birmingham; 1993.
- 29 Pellechia PJ,Gao JX,Gu YL,Ploehn HJ,Murphy CJ. Platinum ion uptake by dendrimers: An NMR and AFM study. Inorg Chem 2004; 43: 1421–1428.
- 30 Donath K. Preparation of histologic sections by cutting-grinding technique for hard tissue and other materials not suitable to be sectioned by routine methods. Norderstedt: Exakt-Kulzer; 1993. p 1–16.
- 31 Hallgren C,Sawase T,Ortengren U,Wennerberg A. Histomorphometric and mechanical evaluation of the bone-tissue response to implants prepared with different orientation of surface topography. Clin Implant Dent Relat Res 2001; 3: 194–203.
- 32 Jager C,Welzel T,Meyer-Zaika W,Epple M. A solid-state NMR investigation of the structure of nanocrystalline hydroxyapatite. Magn Reson Chem 2006; 44: 573–580.
- 33 Cook SD,Thomas KA,Dalton JE,Volkman TK,Whitecloud TSIII,Kay JF. Hydroxylapatite coating of porous implants improves bone ingrowth and interface attachment strength. J Biomed Mater Res 1992; 26: 989–1001.
- 34 Gottlander M,Albrektsson T. Histomorphometric studies of hydroxylapatite-coated and uncoated CP titanium threaded implants in bone. Int J Oral Maxillofac Impl 1991; 6: 399–404.
- 35 Jarcho M. Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop Relat Res 1981: 259–278.
- 36 LeGeros RZ. Biodegradation and bioresorption of calcium phosphate ceramics. Clin Mater 1993; 14: 65–88.
- 37 de Oliveira PT,Nanci A. Nanotexturing of titanium-based surfaces upregulates expression of bone sialoprotein and osteopontin by cultured osteogenic cells. Biomaterials 2004; 25: 403–413.
- 38 Albrektsson T,Wennerberg A. Oral implant surfaces, Part 1: Review focusing on topographic and chemical properties of different surfaces and in vivo responses to them. Int J Prosthodont 2004; 17: 536–543.
- 39
Wennerberg A,Albrektsson T,Lausmaa J.
Torque and histomorphometric evaluation of c.p. titanium screws blasted with 25- and 75-microns-sized particles of Al2O3.
J Biomed Mater Res
1996;
30:
251–260.
10.1002/(SICI)1097-4636(199602)30:2<251::AID-JBM16>3.0.CO;2-P CAS PubMed Web of Science® Google Scholar
- 40 Sul YT,Johansson CB,Roser K,Albrektsson T. Qualitative and quantitative observations of bone tissue reactions to anodised implants. Biomaterials 2002; 23: 1809–1817.
- 41 Wennerberg A,Albrektsson T,Johansson C,Andersson B. Experimental study of turned and grit-blasted screw-shaped implants with special emphasis on effects of blasting material and surface topography. Biomaterials 1996; 17: 15–22.
- 42 Rice JM,Hunt JA,Gallagher JA,Hanarp P,Sutherland DS,Gold J. Quantitative assessment of the response of primary derived human osteoblasts and macrophages to a range of nanotopography surfaces in a single culture model in vitro. Biomaterials 2003; 24: 4799–4818.
- 43 Meirelles L,Currie F,Jacobsson M,Albrektsson T,Wennerberg A. The effect of chemical and nanotopographical modifications on early stage of osseointegration. Int J Oral Maxillofac Surg. Submitted for publication. Int J Oral Maxillofac Implants. Forthcoming.
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