STRUCTURE OF TOTAL BARLEY BETA-GLUCAN1
M. J. Edney
Grain Research Laboratory, Canadian Grain Commission, 1404–303 Main Street, Winnipeg, Manitoba, Canada, R3C 3G8
Search for more papers by this authorB. A. Marchylo
Grain Research Laboratory, Canadian Grain Commission, 1404–303 Main Street, Winnipeg, Manitoba, Canada, R3C 3G8
Search for more papers by this authorA. W. MacGregor
Grain Research Laboratory, Canadian Grain Commission, 1404–303 Main Street, Winnipeg, Manitoba, Canada, R3C 3G8
Paper No. 646 of the Canadian Grain Commission, Grain Research Laboratory, 1404–303 Main Street, Winnipeg, Manitoba, R3C 3G8, Canada.
Search for more papers by this authorM. J. Edney
Grain Research Laboratory, Canadian Grain Commission, 1404–303 Main Street, Winnipeg, Manitoba, Canada, R3C 3G8
Search for more papers by this authorB. A. Marchylo
Grain Research Laboratory, Canadian Grain Commission, 1404–303 Main Street, Winnipeg, Manitoba, Canada, R3C 3G8
Search for more papers by this authorA. W. MacGregor
Grain Research Laboratory, Canadian Grain Commission, 1404–303 Main Street, Winnipeg, Manitoba, Canada, R3C 3G8
Paper No. 646 of the Canadian Grain Commission, Grain Research Laboratory, 1404–303 Main Street, Winnipeg, Manitoba, R3C 3G8, Canada.
Search for more papers by this authorAbstract
The fine structure of total barley β-glucan, as extracted by hot perchloric acid, was investigated by partial enzymatic hydrolysis. Molecular weight profiles of the resulting oligomeric products were similar to those from hydrolysed 40°C water-soluble β-glucan. Concentrations of individual oligosaccharides from total β-glucan were found to vary between oats and barley and among barley varieties, suggesting variability in β-glucan structure. Methylation studies, using HPLC to separate methylated sugars, showed no evidence for the presence of contiguous β-1,3 links in total barley β-glucan, although not all fractions of total β-glucan were analysed.
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