Transfection of glioma cells with the neural-cell adhesion molecule NCAM: Effect on glioma-cell invasion and growth in vivo
Corresponding Author
Klaus Edvardsen
Research Center for Medical Biotechnology, Protein Laboratory, University of Copenhagen, Panum Institute, DK-2200 Copenhagen, Denmark
Research Center for Medical Biotechnology, Protein Laboratory, University of Copenhagen, Panum Institute, DK-2200 Copenhagen, DenmarkSearch for more papers by this authorPaal-Henning Pedersen
The Gade Institute, Department of Pathology, University of Bergen, N-5021 Haukeland Hospital, Bergen, Norway
Search for more papers by this authorRolf Bjerkvig
The Gade Institute, Department of Pathology, University of Bergen, N-5021 Haukeland Hospital, Bergen, Norway
Department of Anatomy and Cell Biology, University of Bergen, Bergen, Norway
Search for more papers by this authorGregers G. Hermann
Department of Tumor Cell Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark
Search for more papers by this authorJesper Zeuthen
Department of Tumor Cell Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark
Search for more papers by this authorOle Didrik Laerum
The Gade Institute, Department of Pathology, University of Bergen, N-5021 Haukeland Hospital, Bergen, Norway
Search for more papers by this authorFrank S. Waish
Department of Experimental Pathology, UMDS, Guy's Hospital, London Bridge, London SE1 9RT, UK
Search for more papers by this authorElisabeth Bock
Research Center for Medical Biotechnology, Protein Laboratory, University of Copenhagen, Panum Institute, DK-2200 Copenhagen, Denmark
Search for more papers by this authorCorresponding Author
Klaus Edvardsen
Research Center for Medical Biotechnology, Protein Laboratory, University of Copenhagen, Panum Institute, DK-2200 Copenhagen, Denmark
Research Center for Medical Biotechnology, Protein Laboratory, University of Copenhagen, Panum Institute, DK-2200 Copenhagen, DenmarkSearch for more papers by this authorPaal-Henning Pedersen
The Gade Institute, Department of Pathology, University of Bergen, N-5021 Haukeland Hospital, Bergen, Norway
Search for more papers by this authorRolf Bjerkvig
The Gade Institute, Department of Pathology, University of Bergen, N-5021 Haukeland Hospital, Bergen, Norway
Department of Anatomy and Cell Biology, University of Bergen, Bergen, Norway
Search for more papers by this authorGregers G. Hermann
Department of Tumor Cell Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark
Search for more papers by this authorJesper Zeuthen
Department of Tumor Cell Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark
Search for more papers by this authorOle Didrik Laerum
The Gade Institute, Department of Pathology, University of Bergen, N-5021 Haukeland Hospital, Bergen, Norway
Search for more papers by this authorFrank S. Waish
Department of Experimental Pathology, UMDS, Guy's Hospital, London Bridge, London SE1 9RT, UK
Search for more papers by this authorElisabeth Bock
Research Center for Medical Biotechnology, Protein Laboratory, University of Copenhagen, Panum Institute, DK-2200 Copenhagen, Denmark
Search for more papers by this authorAbstract
The tumor growth and the invasive capacity of a rat glioma cell line (BT4Cn) were studied after transfection with the human transmembrane 140-kDa isoform of the neural-cell adhesion molecule, NCAM. After s.c. injection, the NCAM-transfected cells showed a slower growth rate than the parent cell line (BT4Cn). Upon intracerebral implantation with BT4Cn cells and different clones of NCAM-transfected cells, all animals developed neurological symptoms within 13-16 days. However, the tumors showed different growth characteristics. The NCAM-transfected BT4Cn cells were localized in the region of the injection site, with a sharply demarcated border between the tumor and brain tissue. In contrast, the parental cell line showed single-cell infiltration and more pronounced destruction of normal brain tissue. Using a 51Cr-release assay, spleen cells from rats transplanted with BT4Cn tumor cells generally showed a lower cytotoxic response than the spleen cells from rats transplanted with the transfected variants of BT4Cn cells, indicating that the transfection procedure in itself mediated an activation of the immune system. The present data suggest that NCAM may influence the malignant behavior of rat glioma cells in vivo. © 1994 Wiley-Liss, Inc.
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