Article

Full Access

Detection of small-cell-lung-cancer cells in bone-marrow aspirates by monoclonal antibodies NCC-LU-243, NCC-LU-246 and MLuCl

Corresponding Author

F. Pasini

Divisione e Cattedra di Oncologia Medica, Università di Verona, Piazzale Stefani, 1, Verona

Divisione e Cattedra di Oncologia Medica, Università di Verona, Piazzale Stefani, 1, Verona. Fax: 45-8341277Search for more papers by this author

G. Pelosi,

G. Pelosi

Servizio di Anatomia Patologica, Ospedale Civile Maggiore, Verona, Italy

Search for more papers by this author

J. A. Ledermann,

J. A. Ledermann

Department of Oncology, University College London Medical School, London W1P8BT, UK

Search for more papers by this author

G. L. Cetto,

G. L. Cetto

Divisione e Cattedra di Oncologia Medica, Università di Verona, Piazzale Stefani, 1, Verona

Search for more papers by this author

F. Pasini,

Corresponding Author

F. Pasini

Divisione e Cattedra di Oncologia Medica, Università di Verona, Piazzale Stefani, 1, Verona

Divisione e Cattedra di Oncologia Medica, Università di Verona, Piazzale Stefani, 1, Verona. Fax: 45-8341277Search for more papers by this author

G. Pelosi,

G. Pelosi

Servizio di Anatomia Patologica, Ospedale Civile Maggiore, Verona, Italy

Search for more papers by this author

J. A. Ledermann,

J. A. Ledermann

Department of Oncology, University College London Medical School, London W1P8BT, UK

Search for more papers by this author

G. L. Cetto,

G. L. Cetto

Divisione e Cattedra di Oncologia Medica, Università di Verona, Piazzale Stefani, 1, Verona

Search for more papers by this author

First published: 1994

https://doi.org/10.1002/ijc.2910570711

Citations: 9

About

PDF

Tools

Share a link

Email
Wechat
Bluesky

Abstract

We have used immunocytochemistry to identify tumor cells in bone-marrow aspirates of patients with small-cell lung cancer and we have compared the results with conventional histomorphology. The monoclonal antibodies (MAbs) used were NCC-LU-243 and NCC-LU-246 (cluster 1) and MLuCl (cluster 6); 108 slides (52 patients) incubated with NCC-LU-246, 106 (51 patients) with NCC-LU-243 and 69 with MLuCl (36 patients) were evaluated. Bone-marrow biopsies (83) were also obtained from 50 of these patients; 48/108 bone marrow aspirates were positive for cluster-1 antigen and 22/69 were positive for MLuCl. Immunocytochemistry with anti-NCAM MAbs detected bone-marrow localization in 38/83 bone-marrow aspirates compared with 9/83 bone-marrow biopsies, while MLuCl was positive in 18/60 and bone-marrow biopsies in 6/60. Moreover, bone-marrow aspirates were positive for cluster-1 antigen in 7/17 patients with limited disease at diagnosis. Patients with positive bone-marrow biopsies had significantly shorter survival. No differences were found between patients with positive and those with negative marrow aspirates.

References

Beiske, K., Myklebust, T., Aamdal, S., Langholm, R., Jakobsen, E. and Fodstad, O., Detection of bone-marrow metastasis in small-cell-lung-cancer patients. Amer. J. Pathol., 141, 531–538 (1992).
CAS PubMed Web of Science® Google Scholar
Berendsen, H. H., De Leij, L., Postmus, P. E., Ter Haar, J. G., Poppema, S. and The, T. H., Detection of small-cell-lung-cancer metastasis in bone-marrow aspirates using monoclonal antibody directed against neuro-endocrine differentation antigen. J. clin. Pathol., 41, 273–276 (1988).
10.1136/jcp.41.3.273
CAS PubMed Web of Science® Google Scholar
Campos-Filmo, N. and Franco, E. L., Microcomputer-assisted univariated survival data analysis using Kaplan-Meier life-table estimators. Comp. Meth. Progr. Biomed., 27, 223–228 (1988).
10.1016/0169-2607(88)90086-7
Web of Science® Google Scholar
Hay, F. G., Ford, A. and Leonard, C. F., Clinical application of immunocytochemistry in the monitoring of the bone marrow in small-cell lung cancer (SCLC). Int. J. Cancer, 2, 8–10 (1988).
10.1002/ijc.2910410704
CAS PubMed Google Scholar
Hirano, T., Hirohashi, S., Kunii, T., Noguchi, M., Shimosato, Y. and Hayata, Y., Quantitative distribution of cluster-1 small-cell-lung-cancer antigen in cancerous and non-cancerous tissues, cultured cells and sera. Jap. J. Cancer Res., 80, 348–355 (1989).
10.1111/j.1349-7006.1989.tb02318.x
CAS PubMed Web of Science® Google Scholar
Hirsch, F. R. and Hansen, H. H., Bone-marrow involvement in small-cell anaplastic carcinoma of the lung. Prognostic and therapeutic aspects. Cancer, 46, 206–211 (1980).
10.1002/1097-0142(19800701)46:1<206::AID-CNCR2820460134>3.0.CO;2-A
CAS PubMed Web of Science® Google Scholar
Hirsch, F., Hansen, H. H., Dombernowsky, P. and Hainau, B., Bone-marrow examination in the staging of small-cell anaplastic carcinoma of the lung with special reference to subtyping. An evaluation of 203 consecutive patients. Cancer, 39, 2563–2567 (1977).
10.1002/1097-0142(197706)39:6<2563::AID-CNCR2820390638>3.0.CO;2-I
CAS PubMed Web of Science® Google Scholar
Kelly, B. W., Morris, J. F., Harwood, B. P. and Bruya, T. E., Methods and prognostic value of bone-marrow examination in small-cell carcinoma of the lung. Cancer, 53, 99–102 (1984).
10.1002/1097-0142(19840101)53:1<99::AID-CNCR2820530118>3.0.CO;2-Z
CAS PubMed Web of Science® Google Scholar
Leonard, R. C. F., Duncan, L. W. and Hay, F. G., Immunocytological detection of residual marrow disease at clinical remission predicts metastatic relapse in small-cell lung cancer. Cancer Rs., 50, 6545–6548 (1990).
CAS PubMed Web of Science® Google Scholar
Ménard, S., Porrow, G., Giani, S., Bedini, A., Bidol, P., Santoro, A., Breghi, M., Gianni, A. M., Pilotti, S., Rilke, F., Soresi, S., Fasolano, S. and Colnaghi, M. I., Detection of SCLC metastatic cells in bone marrow by means of the monoclonal antibody MLuCl. Lung Cancer, 4, 73–75 (1988).
10.1016/S0169-5002(88)80018-7
Google Scholar
Molino, A., Colombatti, M., Bonetti, F., Zardini, M., Pasini, F., Perini, A., Pelosi, G., Tridente, G., Veneri, D. and Cetto, G. L., A comparative analysis of three different techniques for the detection of breast-cancer cells in bone marrow. Cancer, 67, 1033–1036 (1991).
10.1002/1097-0142(19910215)67:4<1033::AID-CNCR2820670428>3.0.CO;2-H
CAS PubMed Web of Science® Google Scholar
Myklebust, A. T., Beiske, K., Pharo, A., de L. Davies, C., Aamdal, S. and Fodstad, O., Selection of anti-SCLC antibodies for diagnosis of bone-marrow metastasis. Brit. J. Cancer, 63 (Suppl. XIV), 49–53 (1991).
Google Scholar
Ripamonti, M., Canevan, S., Ménard, S., Mezzanzanica, D., Miotti, S., Orlandi, R., Rilke, F., Tagleabue, E. and Colnaghi, M., Human carcinoma cell lines xenografted in athymic mice: biological and antigenic characteristics of an intra-abdominal model. Cancer Immunol. Immunother., 24, 13–16 (1987).
10.1007/BF00199827
CAS PubMed Web of Science® Google Scholar
Skov, B. G., Hirsch, F. R. and Bobrow, L. G., Monoclonal antibodies in the detection of bone-marrow metastases in small-cell lung cancer. Brit. J. Cancer, 65, 593–596 (1992).
10.1038/bjc.1992.120
CAS PubMed Web of Science® Google Scholar
Souhami, R. L., Beverly, P. C. L. and Bobrow, L. G., Antigens of small-cell lung cancer. Lancet, II, 325–326 (1987).
10.1016/S0140-6736(87)90904-4
Google Scholar
Souhami, R. L., Beverly, P. C. L., Bobrow, L. G. and Ledermann, J. A., Antigens of lung cancer: results of the second International Workshop on Lung-Cancer Antigens. J. nat. Cancer Inst., 83, 609–612 (1991).
10.1093/jnci/83.9.609
CAS PubMed Web of Science® Google Scholar
Stahel, R. A., Mabry, M., Skarin, A. T., Speak, J. and Bernai, S. D., Detection of bone-marrow metastasis in small-cell lung cancer by monoclonal antibody. J. clin. Oncol., 3, 455–461 (1985).
10.1200/JCO.1985.3.4.455
CAS PubMed Web of Science® Google Scholar
Tome, Y., Hirohashi, S., Noguchi, M., Matsuno, Y., Kishi, K., Uei, Y. and Shimosato, Y., Immunocytologic diagnosis of small-cell lung cancer in imprint smears. Acta Cytol., 35, 485–490 (1991).
CAS PubMed Web of Science® Google Scholar
Trillet, V., Revel, D., Combaret, V., Favrot, M., Loire, R., Tabib, A., Pages, J., Jacquemet, P., Bonmartin, A., Mornex, J. F., Cordier, J. F., Pinet, F., Amiel, O. and Brune, J., Bone-marrow metastasis in small-cell lung cancer; detection with magnetic resonance imaging and monoclonal antibodies. Brit. J. Cancer, 60, 83–88 (1989).
10.1038/bjc.1989.225
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume57, IssueS8

Supplement: Third International IASLC Workshop on Long Tumor and Differentiation Antigens. University Hospital Zurich, Switzerland

1994

Pages 53-56

Detection of small-cell-lung-cancer cells in bone-marrow aspirates by monoclonal antibodies NCC-LU-243, NCC-LU-246 and MLuCl

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Detection of small-cell-lung-cancer cells in bone-marrow aspirates by monoclonal antibodies NCC-LU-243, NCC-LU-246 and MLuCl

Abstract

References

Citing Literature

References

Related

Information