Characterization of an embryonal rhabdomyosarcoma cell line showing amplification and over-expression of the N-myc oncogene
Yasuhide Hayashi
Saitama Children's Medical Center, Iwatsuki, Saitama 339, Japan
Search for more papers by this authorCorresponding Author
Tohru Sugimoto
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, JapanSearch for more papers by this authorYoshihiro Horii
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Search for more papers by this authorHajime Hosoi
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Search for more papers by this authorJohji Inazawa
Department of Hygiene, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Search for more papers by this authorJohn T. Kemshead
Institute of Child Health, London WC1N 1EH, England
Search for more papers by this authorToshiya Inaba
Saitama Children's Medical Center, Iwatsuki, Saitama 339, Japan
Search for more papers by this authorRyoji Hanada
Saitama Children's Medical Center, Iwatsuki, Saitama 339, Japan
Search for more papers by this authorKeiko Yamamoto
Saitama Children's Medical Center, Iwatsuki, Saitama 339, Japan
Search for more papers by this authorAllen M. Gown
Department of Pathology, University of Washington, Seattle, Washington 98195, USA
Search for more papers by this authorTadashi Sawada
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Search for more papers by this authorYasuhide Hayashi
Saitama Children's Medical Center, Iwatsuki, Saitama 339, Japan
Search for more papers by this authorCorresponding Author
Tohru Sugimoto
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, JapanSearch for more papers by this authorYoshihiro Horii
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Search for more papers by this authorHajime Hosoi
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Search for more papers by this authorJohji Inazawa
Department of Hygiene, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Search for more papers by this authorJohn T. Kemshead
Institute of Child Health, London WC1N 1EH, England
Search for more papers by this authorToshiya Inaba
Saitama Children's Medical Center, Iwatsuki, Saitama 339, Japan
Search for more papers by this authorRyoji Hanada
Saitama Children's Medical Center, Iwatsuki, Saitama 339, Japan
Search for more papers by this authorKeiko Yamamoto
Saitama Children's Medical Center, Iwatsuki, Saitama 339, Japan
Search for more papers by this authorAllen M. Gown
Department of Pathology, University of Washington, Seattle, Washington 98195, USA
Search for more papers by this authorTadashi Sawada
Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
Search for more papers by this authorAbstract
A parent rhabdomyosarcoma cell line designated SCMC-RM2 was established from bone-marrow tumor cells taken from an 11-year-old girl with an embryonal rhabdomyosarcoma. Subsequently a cloned SCMC-RM2-1 cell line was isolated from a parent line. These cell lines grew as adherent monolayers in liquid culture with a doubling time of 50 and 52 hr, respectively. In addition, colonies were established in soft agar, which grew in a dose-dependent fashion with a cloning efficiency of 0.7 and 0.8%, respectively. Chromosomal analysis showed these cell lines had neither double minutes nor homogeneously staining regions. Chromosome number ranged from 61 to 93, translocation; t(9;13)(p22;q14) was identified, and no alteration of chromosome 2 was observed. Surface membrane antigen profile of parent and cloned lines by using a panel of 24 monoclonal antibodies (MAbs) excluded the possibility of these being neuroblastoma cell lines. In addition, MAbs to the cytoplasmic protein desmin, myoglobin, muscle actin (α and γ) and α-sarcomeric actin reacted with these cell lines, SCMC-RM2 and SCMC-RM2-1 being thus identified as rhabdomyosarcoma. Southern blot analyses revealed 8- and 7-fold amplification of the N-myc gene in SCMC-RM2 and SCMC-RM2-1 as compared with the promyelocytic cell line HL60. Over-expression of the N-myc mRNA was noted over control cell lines.
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