Relationship between cellular levels of beta-carotene and sensitivity to genotoxic agents

Hans F. Stick

Environmental Carcinogenesis Unit, British Columbia Cancer Reserch Centre, 601 West 10th Avenue, Vancouver, B.C. V5Z1L3, Canada

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Bruce P. Dunn,

Bruce P. Dunn

Environmental Carcinogenesis Unit, British Columbia Cancer Reserch Centre, 601 West 10th Avenue, Vancouver, B.C. V5Z1L3, Canada

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Hans F. Stick,

Hans F. Stick

Environmental Carcinogenesis Unit, British Columbia Cancer Reserch Centre, 601 West 10th Avenue, Vancouver, B.C. V5Z1L3, Canada

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Bruce P. Dunn,

Bruce P. Dunn

Environmental Carcinogenesis Unit, British Columbia Cancer Reserch Centre, 601 West 10th Avenue, Vancouver, B.C. V5Z1L3, Canada

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First published: 15 November 1986

https://doi.org/10.1002/ijc.2910380515

Citations: 49

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Abstract

The usefulness of an in vitro test system to predict the inhibitory effect of beta-carotene on the genotoxic activity of carcinogens/mutagens was explored. To facilitate the comparison of data obtained from cultured cells (CHO) and from exfoliated human cells, end-points were used which can be quantitated in both cell systems: the frequency of micronuclei for estimating the effect of genotoxic agents, and cellular levels of beta-carotene as a protective agent. In CHO cells, beta-carotene inhibited the clastogenic and micronu-cleus-forming effect of methyl methanesulfonate (MMS) and 4-nitroquinoline-l-oxide (4NQO), but had no protective action against gallic acid, tannic acid, an aqueous extract of areca nut or H₂O₂. The extent of inhibition depended on the ratio of beta-carotene to MMS. Doses of beta-carotene which exerted a protective effect in vitro ranged from approximately 2 to 5 ng per 10⁶ CHO cells. Comparable levels of beta-carotene were previously found to reduce the frequency of micronucleated exfoliated cells from the buccal mu-cosa of tobacco and areca-nut chewers (Stich et al., 1984b).

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Volume38, Issue5

15 November 1986

Pages 713-717

Relationship between cellular levels of beta-carotene and sensitivity to genotoxic agents

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Relationship between cellular levels of beta-carotene and sensitivity to genotoxic agents

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