Volume 38, Issue 5 pp. 631-636

Human Cancer

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Characterization and chromosomal assignment of a human cell surface antigen defined by the monoclonal antibody AUAI

N. K. Spurr,

N. K. Spurr

Human Genetics Resources Laboratory, Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts

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H. Durbin,

H. Durbin

Directors' Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX

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D. Sheer,

D. Sheer

Human Cytogenetics Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX

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M. Parkar,

M. Parkar

MRC Biochemical Genetics Unit, Wolfson House, London

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L. Bobrow,

L. Bobrow

Imperial Cancer Research Fund Laboratory, Department of Histopathology, School of Medicine, University College, London WC1E 6JJ, UK

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W. F. Bodmer,

W. F. Bodmer

Directors' Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX

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N. K. Spurr,

N. K. Spurr

Human Genetics Resources Laboratory, Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts

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H. Durbin,

H. Durbin

Directors' Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX

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D. Sheer,

D. Sheer

Human Cytogenetics Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX

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M. Parkar,

M. Parkar

MRC Biochemical Genetics Unit, Wolfson House, London

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L. Bobrow,

L. Bobrow

Imperial Cancer Research Fund Laboratory, Department of Histopathology, School of Medicine, University College, London WC1E 6JJ, UK

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W. F. Bodmer,

W. F. Bodmer

Directors' Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX

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First published: 15 November 1986

https://doi.org/10.1002/ijc.2910380503

Citations: 63

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Abstract

We describe the chromosomal assignment and biochemical characterization of the genetic locus controlled by a human cell surface antigen which is defined by the monoclonal antibody (MAb) AUAI. This gene product is only expressed on epithelial cells. Therefore, human-mouse somatic cell hybrids of epithelial origin were used to assign this gene to chromosome 2. Cell surface iodination of the hybrids and parental cells followed by immunoprecipitation and polyacrylamide gel electrophoresis showed that AUAI detected a single 35-kDa protein. The MAb AUAI reacted on tissue sections with a subset of normal epithelial cells, but in tumours it showed a much wider distribution, though still only on epithelium-derived tumours.

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Volume38, Issue5

15 November 1986

Pages 631-636

Characterization and chromosomal assignment of a human cell surface antigen defined by the monoclonal antibody AUAI

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Characterization and chromosomal assignment of a human cell surface antigen defined by the monoclonal antibody AUAI

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