Carcinogenesis
B-cells and IL-4 promote methylcholanthrene-induced carcinogenesis but there is no evidence for a role of T/NKT-cells and their effector molecules (Fas-ligand, TNF-α, perforin)
Thomas Kammertoens,
Zhihai Qin,
Dana Briesemeister,
Albert Bendelac,
Thomas Blankenstein,
Thomas Kammertoens
Institut für Immunologie, Charité, Campus Benjamin-Franklin, Berlin, Germany
T.K. and Z.Q. contributed equally to this work
Search for more papers by this authorZhihai Qin
Institut für Immunologie, Charité, Campus Benjamin-Franklin, Berlin, Germany
T.K. and Z.Q. contributed equally to this work
Search for more papers by this authorDana Briesemeister
Institut für Immunologie, Charité, Campus Benjamin-Franklin, Berlin, Germany
Search for more papers by this authorAlbert Bendelac
Department of Pathology, University of Chicago, Chicago, IL
Search for more papers by this authorCorresponding Author
Thomas Blankenstein
Institut für Immunologie, Charité, Campus Benjamin-Franklin, Berlin, Germany
Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany
Tel.: +49-30-94062310, Fax: +49-30-94062453
Max-Delbrück Centrum für Molekulare Medizin, Robert-Rössle Strasse 10, 13122 Berlin, GermanySearch for more papers by this authorThomas Kammertoens,
Zhihai Qin,
Dana Briesemeister,
Albert Bendelac,
Thomas Blankenstein,
Thomas Kammertoens
Institut für Immunologie, Charité, Campus Benjamin-Franklin, Berlin, Germany
T.K. and Z.Q. contributed equally to this work
Search for more papers by this authorZhihai Qin
Institut für Immunologie, Charité, Campus Benjamin-Franklin, Berlin, Germany
T.K. and Z.Q. contributed equally to this work
Search for more papers by this authorDana Briesemeister
Institut für Immunologie, Charité, Campus Benjamin-Franklin, Berlin, Germany
Search for more papers by this authorAlbert Bendelac
Department of Pathology, University of Chicago, Chicago, IL
Search for more papers by this authorCorresponding Author
Thomas Blankenstein
Institut für Immunologie, Charité, Campus Benjamin-Franklin, Berlin, Germany
Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany
Tel.: +49-30-94062310, Fax: +49-30-94062453
Max-Delbrück Centrum für Molekulare Medizin, Robert-Rössle Strasse 10, 13122 Berlin, GermanySearch for more papers by this authorAbstract
Mice deficient either in subtypes of immune cells, cytokines or lytic pathways have been subjected to chemical carcinogenesis by methylcholanthrene to evaluate whether these components of the immune system affect tumor development. Inbred mice of the same genotype but from different sources differed in tumor development in magnitude comparable to that previously attributed to differences in immunocompetence. This suggested that genetic drift between separate inbred colonies of mice and/or environmental factors (e.g., transport of the animals) influenced carcinogenesis. Therefore, littermates were used as control in subsequent experiments. Although deficiency of T-cells, NKT-cells, perforin, Fas-ligand, TNF-α-receptor failed to reveal significant differences in tumor development, the presence of B-cells and IL-4 enhanced tumor development under similar experimental conditions.
Supporting Information
Additional Supporting Information may be found in the online version of this article.
| Filename | Description |
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| IJC_27411_sm_SuppFigs.pdf83.7 KB | Supporting Information Figure 1. BALB/c nude and non-littermates as indicated were injected with MCA and tumor development was observed. Amount and injection route of MCA, number of mice per experimental group (in parenthesis) and sex of the mice are indicated. A), B) 800 μg, C), D) 400 μg MCA. E) C57 BL/6 nude (nu/nu), heterozygous (nu/wt) and homozygous (wt/wt) control littermates. Figure 2. B-cell deficiency increases latency after MCA-carcinogenesis. B-cell-deficient μMT mice (??) and control littermates (WT) as indicated were injected with 800μg MCA and tumor development was observed. Amount and injection route of MCA, number of mice per experimental group (in parenthesis) and sex of the mice are indicated. Figure 3. IL-6 or IL-10 deficiency does not affect MCA-carcinogenesis at high doses of MCA. IL-6 (A-B) or IL-10 (C-D)-deficient mice and control littermates (WT) as indicated were injected with MCA and tumor development was observed. Amount and injection route of MCA, number of mice per experimental group (in parenthesis) and sex of the mice are indicated. |
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