Intraepithelial p63-dependent expression of distinct components of cell adhesion complexes in normal esophageal mucosa and squamous cell carcinoma
Corresponding Author
Amélie Thépot
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Banque de Tissus et cellules, Hôpital E. Herriot, Lyon, France
Tel.: 33-4-72-73-84-62, Fax: 33-4-72-73-83-22
Group of Molecular Carcinogenesis and Biomarkers, IARC, 150 cours Albert Thomas, 69372 Lyon Cedex 08, FranceSearch for more papers by this authorAgnès Hautefeuille
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorMarie-Pierre Cros
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorBehnoush Abedi-Ardekani
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorAurélia Pétré
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorOdile Damour
Banque de Tissus et cellules, Hôpital E. Herriot, Lyon, France
Search for more papers by this authorVladimir Krutovskikh
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorCorresponding Author
Pierre Hainaut
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Tel.: 33-4-72-73-84-62, Fax: 33-4-72-73-83-22
Group of Molecular Carcinogenesis and Biomarkers, IARC, 150 cours Albert Thomas, 69372 Lyon Cedex 08, FranceSearch for more papers by this authorCorresponding Author
Amélie Thépot
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Banque de Tissus et cellules, Hôpital E. Herriot, Lyon, France
Tel.: 33-4-72-73-84-62, Fax: 33-4-72-73-83-22
Group of Molecular Carcinogenesis and Biomarkers, IARC, 150 cours Albert Thomas, 69372 Lyon Cedex 08, FranceSearch for more papers by this authorAgnès Hautefeuille
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorMarie-Pierre Cros
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorBehnoush Abedi-Ardekani
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorAurélia Pétré
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorOdile Damour
Banque de Tissus et cellules, Hôpital E. Herriot, Lyon, France
Search for more papers by this authorVladimir Krutovskikh
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Search for more papers by this authorCorresponding Author
Pierre Hainaut
Molecular Carcinogenesis Group and Epigenetics Group, IARC, Lyon, France
Tel.: 33-4-72-73-84-62, Fax: 33-4-72-73-83-22
Group of Molecular Carcinogenesis and Biomarkers, IARC, 150 cours Albert Thomas, 69372 Lyon Cedex 08, FranceSearch for more papers by this authorAbstract
TP63 gene is a member of TP53 tumor suppressor gene family that encodes several protein isoforms involved in the process of epithelial stratification and in epithelial-mesenchyme interactions. TP63 is amplified in a significant proportion of squamous cell carcinoma of the esophagus (ESCC), resulting in the hyper-expression of ΔNp63 as the major p63 isoform. To better understand the contribution of this high expression to tumorigenesis, we have analyzed the impact of intraepithelial p63 expression on the expression of cell adhesion complexes in normal esophagus and in ESCC cell lines. Cells expressing p63 showed an adhesion pattern characterized by lack of tight junctions and presence of adherens junctions. Cell differentiation was accompanied by a decrease in p63 and by a shift to adhesion patterns involving tight junctions. Silencing of p63 mRNA in ESCC cell lines resulted in a similar shift, characterized by increased expression of component of tight junctions, decreased cell-to-cell communication and downregulation of cell proliferation. These results indicate that ΔNp63 may contribute to esophageal squamous carcinogenesis by maintaining cell adhesion patterns compatible with cell proliferation.
Supporting Information
Additional Supporting Information may be found in the online version of this article
| Filename | Description |
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| IJC_25221_sm_suppfig1.tif577.5 KB | Supporting Figure 1 |
| IJC_25221_sm_suppfig2.tif339.1 KB | Supporting Figure 2 |
| IJC_25221_sm_suppfig3.tif55 KB | Supporting Figure 3 |
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