Biases in human papillomavirus genotype prevalence assessment associatedwith commonly used consensus primers†‡
Corresponding Author
Paul K. S. Chan
School of Public Health, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Fax: +852-2647-3227
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, ChinaSearch for more papers by this authorTak-Hong Cheung
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital,Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorAnn O. Y. Tam
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorKeith W. K. Lo
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital,Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorSo-Fan Yim
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital,Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorMay M. Y. Yu
Department of Anatomical and Cellular Pathology, Faculty of Medicine, The Chinese University of Hong Kong,Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorKa-Fai To
Department of Anatomical and Cellular Pathology, Faculty of Medicine, The Chinese University of Hong Kong,Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorYick-Fu Wong
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital,Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorJo L. K. Cheung
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorDenise P. C. Chan
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorMamie Hui
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorMargaret Ip
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorCorresponding Author
Paul K. S. Chan
School of Public Health, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Fax: +852-2647-3227
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, ChinaSearch for more papers by this authorTak-Hong Cheung
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital,Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorAnn O. Y. Tam
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorKeith W. K. Lo
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital,Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorSo-Fan Yim
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital,Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorMay M. Y. Yu
Department of Anatomical and Cellular Pathology, Faculty of Medicine, The Chinese University of Hong Kong,Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorKa-Fai To
Department of Anatomical and Cellular Pathology, Faculty of Medicine, The Chinese University of Hong Kong,Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorYick-Fu Wong
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital,Shatin, New Territories, Hong Kong SAR, China
Search for more papers by this authorJo L. K. Cheung
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorDenise P. C. Chan
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorMamie Hui
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorMargaret Ip
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China
Search for more papers by this authorThis study was presented in the 22nd International Papillomavirus Conference in Vancouver, 2–6 May 2005.
The authors do not have a commercial or other association that might pose a conflict of interest.
Abstract
Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) in parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor sensitivity for HPV52, for which the prevalence among squamous cell cervical cancer was underestimated from 14.6% to 0%. The fact that HPV52 should rank second in prevalence among squamous cell cervical carcinoma in Hong Kong could be missed if GP5+/6+, a worldwide commonly used primer set, was selected for HPV detection. Biases in HPV type-specific sensitivity may result in misprioritization of vaccine candidates. © 2005 Wiley-Liss, Inc.
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