Diagnostic value of exosomal circMYC in radioresistant nasopharyngeal carcinoma
Yanwei Luo PhD
Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorJinqi Ma MD
Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorFengxia Liu MD
Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorCorresponding Author
Jie Guo PhD
National Institution of Drug Clinical Trial, Xiangya Hospital, Central South University, Changsha, China
Correspondence
Jie Guo, National Institution of Drug Clinical Trial, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, Hunan, China.
Email: [email protected]
Rong Gui, Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Tongzipo Road 138, Changsha 410013, Hunan, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Rong Gui PhD
Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, China
Correspondence
Jie Guo, National Institution of Drug Clinical Trial, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, Hunan, China.
Email: [email protected]
Rong Gui, Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Tongzipo Road 138, Changsha 410013, Hunan, China.
Email: [email protected]
Search for more papers by this authorYanwei Luo PhD
Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorJinqi Ma MD
Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorFengxia Liu MD
Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorCorresponding Author
Jie Guo PhD
National Institution of Drug Clinical Trial, Xiangya Hospital, Central South University, Changsha, China
Correspondence
Jie Guo, National Institution of Drug Clinical Trial, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, Hunan, China.
Email: [email protected]
Rong Gui, Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Tongzipo Road 138, Changsha 410013, Hunan, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Rong Gui PhD
Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, China
Correspondence
Jie Guo, National Institution of Drug Clinical Trial, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, Hunan, China.
Email: [email protected]
Rong Gui, Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Tongzipo Road 138, Changsha 410013, Hunan, China.
Email: [email protected]
Search for more papers by this authorFunding information: National Natural Science Foundation of China, Grant/Award Numbers: 81573091, 81802668; Natural Science Foundation of Hunan Province, Grant/Award Numbers: 2017JJ3467, 2018JJ3776
Abstract
Background
The relationship between circulating exosomal circular RNA (circRNA) and prognosis of patients with nasopharyngeal carcinoma (NPC) remain unknown. This study focused on the expression of exosomal circMYC and its relationship with the recurrence and prognosis of patients with NPC.
Methods
The circulating exosomes were obtained from 210 patients with NPC. Quantitative polymerase chain reaction, 5-ethynyl-2′-deoxyuridine (EdU) staining, colony formation, and bioinformatic analysis were performed.
Results
Circulating exosomal circMYC was significantly increased in patients with NPC and was associated with tumor size, lymph node metastasis, TNM stage, survival rate, and disease recurrence. Gain-functional and loss-functional experiments revealed that overexpression of circMYC promoted cell proliferation and reduce radiosensitivity, while knockdown of circMYC inhibited cell proliferation and enhanced radiotherapy.
Conclusion
circMYC is an oncogene in NPC cells and can enhance the radiotherapy resistance of NPC cells. Circulating exosomal circMYC can be used as a potential therapeutic target for NPC.
CONFLICT OF INTEREST
The authors declare no potential conflict of interest.
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