Highly restricted expression of Cre recombinase in cerebellar Purkinje cells
Xin-Mei Zhang
Department of Biochemistry, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Search for more papers by this authorAlam Hoi-Lam Ng
Department of Biochemistry, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Search for more papers by this authorJulian A. Tanner
Department of Biochemistry, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Search for more papers by this authorWu-Tian Wu
Department of Anatomy, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Search for more papers by this authorNeal G. Copeland
Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland
Search for more papers by this authorNancy A. Jenkins
Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland
Search for more papers by this authorCorresponding Author
Jian-Dong Huang
Department of Biochemistry, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Department of Biochemistry, the University of Hong Kong, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, ChinaSearch for more papers by this authorXin-Mei Zhang
Department of Biochemistry, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Search for more papers by this authorAlam Hoi-Lam Ng
Department of Biochemistry, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Search for more papers by this authorJulian A. Tanner
Department of Biochemistry, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Search for more papers by this authorWu-Tian Wu
Department of Anatomy, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Search for more papers by this authorNeal G. Copeland
Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland
Search for more papers by this authorNancy A. Jenkins
Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland
Search for more papers by this authorCorresponding Author
Jian-Dong Huang
Department of Biochemistry, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China
Department of Biochemistry, the University of Hong Kong, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, ChinaSearch for more papers by this authorAbstract
The Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation. genesis 40:45–51, 2004. © 2004 Wiley-Liss, Inc.
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