Characterization of a t(I0; II) (pI3-I4; qI4-2I) in the monoblastic cell line U937
Corresponding Author
Janet Shipley
Cell Biology and Experimental Pathology, Institute of Cancer Research, Belmont, Sutton, Surrey
Molecular Cytogenetics Laboratory, Institute of Cancer Research, F-Block, 15 Cotswold Road, Belmont, Sutton, Surrey, SMZ 5NG United KingdomSearch for more papers by this authorAnne O'Byrne
Imperial Cancer Research Fund, and Academic Department of Haematology and Cytogenetics, Royal Marsden Hospital and Institute of Cancer Research, London, United Kingdom
Search for more papers by this authorLyndal Kearney
MRC Molecular Haematology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom
Search for more papers by this authorBryan Young
Department of Medical Oncology, St. Bartholomew's Hospital, London, United Kingdom
Search for more papers by this authorMartin Dyer
Imperial Cancer Research Fund, and Academic Department of Haematology and Cytogenetics, Royal Marsden Hospital and Institute of Cancer Research, London, United Kingdom
Search for more papers by this authorDaniel Catovsky
Imperial Cancer Research Fund, and Academic Department of Haematology and Cytogenetics, Royal Marsden Hospital and Institute of Cancer Research, London, United Kingdom
Search for more papers by this authorBarry Gusterson
Cell Biology and Experimental Pathology, Institute of Cancer Research, Belmont, Sutton, Surrey
Search for more papers by this authorCorresponding Author
Janet Shipley
Cell Biology and Experimental Pathology, Institute of Cancer Research, Belmont, Sutton, Surrey
Molecular Cytogenetics Laboratory, Institute of Cancer Research, F-Block, 15 Cotswold Road, Belmont, Sutton, Surrey, SMZ 5NG United KingdomSearch for more papers by this authorAnne O'Byrne
Imperial Cancer Research Fund, and Academic Department of Haematology and Cytogenetics, Royal Marsden Hospital and Institute of Cancer Research, London, United Kingdom
Search for more papers by this authorLyndal Kearney
MRC Molecular Haematology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom
Search for more papers by this authorBryan Young
Department of Medical Oncology, St. Bartholomew's Hospital, London, United Kingdom
Search for more papers by this authorMartin Dyer
Imperial Cancer Research Fund, and Academic Department of Haematology and Cytogenetics, Royal Marsden Hospital and Institute of Cancer Research, London, United Kingdom
Search for more papers by this authorDaniel Catovsky
Imperial Cancer Research Fund, and Academic Department of Haematology and Cytogenetics, Royal Marsden Hospital and Institute of Cancer Research, London, United Kingdom
Search for more papers by this authorBarry Gusterson
Cell Biology and Experimental Pathology, Institute of Cancer Research, Belmont, Sutton, Surrey
Search for more papers by this authorAbstract
Previous analysis of the monoblastic cell line U937 has shown that several sublines contain a rearranged chromosome arm I I q. In order to determine the true nature of the rearrangement, fluorescence in situ hybridization (FISH) was carried out with various combinations of single copy anonymous markers, clones containing genes, a chromosome 10 paint, and an I I centromere specific sequence. The rearrangement was deduced to be a reciprocal translocation between chromosomes 10 and 11 described as t( 10; 11 )(p 13- 14;q 14-21 ). The breakpoint on chromosome 11 is telomeric to the /NT2 gene and the pHS11 probe at 11 q 13, and centromeric to the marker D 11536 localized to 11q 14.3-q22. 1 and the MLL gene at 11 q23. Similar translocations have been reported in various acute leukemias, principally of the monocytic lineage, and also in T-cell precursor acute lymphocytic leukemias. Further characterization of the genetic rearrangements in U937 may lead to the isolation of genes important in leukemogenesis and provide an in vitro system for their study. © 1995 Wiley-Liss, Inc.
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