Detection of somatic changes in human renal cell carcinomas with oligonucleotide probes specific for simple repeat motifs
Susanne Bock
Institut für klinische Hämatologie, Forschungszentrum für Umwelt und Gesundheit GmbH, Hämatologikum, München, Germany
Search for more papers by this authorJörg T. Epplen
Molekulare Humangenetik der Ruhr-Universität, Bochum, Germany
Search for more papers by this authorHeidi Noll-Puchta
Molekulare Onkologie, Medizinische Klinik III. Klinikum Großhadern, München, Germany
Search for more papers by this authorMichael Rotter
Institut für Allgemeine Pathologie und Pathologische Anatomie der Technischen Universität München, Klinikum rechts der lsar, München, Germany
Search for more papers by this authorHeinz Höfler
Institut für Allgemeine Pathologie und Pathologische Anatomie der Technischen Universität München, Klinikum rechts der lsar, München, Germany
Search for more papers by this authorThomas Block
Urologische Klinik und Poliklinik der Technischen Universität München, Klinikum rechts der Isar, München, Germany
Search for more papers by this authorRudolph Hartung
Urologische Klinik und Poliklinik der Technischen Universität München, Klinikum rechts der Isar, München, Germany
Search for more papers by this authorGerhard Jakse
Urologische Klinik der medizinischen Fakultät der RWTH Aachen, Aachen, Germany
Search for more papers by this authorWolfgang Wilmanns
Institut für klinische Hämatologie, Forschungszentrum für Umwelt und Gesundheit GmbH, Hämatologikum, München, Germany
Molekulare Onkologie, Medizinische Klinik III. Klinikum Großhadern, München, Germany
Search for more papers by this authorCorresponding Author
Petro E. Petrides
Institut für klinische Hämatologie, Forschungszentrum für Umwelt und Gesundheit GmbH, Hämatologikum, München, Germany
Molekulare Onkologie, Medizinische Klinik III. Klinikum Großhadern, München, Germany
Institut für Klinische Hämatologie, Forschungszentrum für Umwelt und Gesundheit GmbH, Hämatologikum, Marchioninistr. 25, W-8000 München 70, GermanySearch for more papers by this authorSusanne Bock
Institut für klinische Hämatologie, Forschungszentrum für Umwelt und Gesundheit GmbH, Hämatologikum, München, Germany
Search for more papers by this authorJörg T. Epplen
Molekulare Humangenetik der Ruhr-Universität, Bochum, Germany
Search for more papers by this authorHeidi Noll-Puchta
Molekulare Onkologie, Medizinische Klinik III. Klinikum Großhadern, München, Germany
Search for more papers by this authorMichael Rotter
Institut für Allgemeine Pathologie und Pathologische Anatomie der Technischen Universität München, Klinikum rechts der lsar, München, Germany
Search for more papers by this authorHeinz Höfler
Institut für Allgemeine Pathologie und Pathologische Anatomie der Technischen Universität München, Klinikum rechts der lsar, München, Germany
Search for more papers by this authorThomas Block
Urologische Klinik und Poliklinik der Technischen Universität München, Klinikum rechts der Isar, München, Germany
Search for more papers by this authorRudolph Hartung
Urologische Klinik und Poliklinik der Technischen Universität München, Klinikum rechts der Isar, München, Germany
Search for more papers by this authorGerhard Jakse
Urologische Klinik der medizinischen Fakultät der RWTH Aachen, Aachen, Germany
Search for more papers by this authorWolfgang Wilmanns
Institut für klinische Hämatologie, Forschungszentrum für Umwelt und Gesundheit GmbH, Hämatologikum, München, Germany
Molekulare Onkologie, Medizinische Klinik III. Klinikum Großhadern, München, Germany
Search for more papers by this authorCorresponding Author
Petro E. Petrides
Institut für klinische Hämatologie, Forschungszentrum für Umwelt und Gesundheit GmbH, Hämatologikum, München, Germany
Molekulare Onkologie, Medizinische Klinik III. Klinikum Großhadern, München, Germany
Institut für Klinische Hämatologie, Forschungszentrum für Umwelt und Gesundheit GmbH, Hämatologikum, Marchioninistr. 25, W-8000 München 70, GermanySearch for more papers by this authorAbstract
The purpose of our study was to detect somatic changes in renal cell carcinoma by multilocus fingerprinting. DNA fingerprints were generated from the DNA of normal and malignant renal tissue samples of 29 patients with nonhereditary kidney carcinoma by using oligonucleotide probes specific for simple repeat motifs such as (GTG)5, (CA)8, (GACA)4, or (TTAGGG)3. Each probe resdered a typical fingerprint pattern, because it is specific with respect to the target regions recognized in the genome. The restriction enzymes used were Hinfl and HaeIII. Changed banding patterns were detected by using (GTG)5 in 20% of the tumors, in 20% for (CA)8 after Hinfl digestion, and in 10% after HaeIII digestion. Even more informative probes were (GACA)4, showing 70% changes after HaeIII digestion, and (TTAGGG)3, with 80% changes after digestion with either enzyme. Since the simple repeat motifs recognized by (GACA)4 are localized on the short arms of the acrocentric chromosomes (13, 14, 15, 21, and 22), it is possible that sequences important for renal carcinogenesis are present in these regions. The observation of changes in regions to which (TTAGGG)3 hybridizes points to an involvement of DNA elements in telomeric sequence related regions in human kidney tumor formation. © 1993 Wiley-Liss, Inc.
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