Interleukin-15 preferentially promotes the growth of intestinal intraepithelial lymphocytes bearing γδ T cell receptor in mice
Kyoko Inagaki-Ohara
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan
Search for more papers by this authorHitoshi Nishimura
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan
Search for more papers by this authorAkio Mitani
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan
Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Search for more papers by this authorCorresponding Author
Yasunobu Yoshikai
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho Showa-ku Nagoya 466, Japan Fax: +81-52-744-2449Search for more papers by this authorKyoko Inagaki-Ohara
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan
Search for more papers by this authorHitoshi Nishimura
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan
Search for more papers by this authorAkio Mitani
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan
Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Search for more papers by this authorCorresponding Author
Yasunobu Yoshikai
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho Showa-ku Nagoya 466, Japan Fax: +81-52-744-2449Search for more papers by this authorAbstract
Several cytokines including stem cell factor (SCF) and interleukin (IL)-7 are known to be required for development of γδ T cell receptor (TCR) intestinal intraepithelial lymphocytes (i-IEL) in mice. We show here the effects of IL-15 on the proliferation and maintenance of murine γδ i-IEL in vitro. γδ i-IEL constitutively expressed a high level of IL-15 receptor α mRNA and proliferated in response to IL-15 more vigorously than αβ i-IEL. Vγ/δ repertoire analysis revealed that IL-15, like IL-2, induced polyclonal expansion of γδ i-IEL, whereas γδ i-IEL responding to IL-7 showed a Vγ/δ repertoire skewed towards Vγ1/Vδ4, Vδ5. IL-15 efficiently prevented γδ i-IEL from apoptosis induced by growth factor deprivation. This rescue was accompanied by up-regulation of Bcl-2 expression. These results suggest that IL-15 plays important roles in proliferation and maintenance of γδ i-IEL.
References
- 1 Lefrançois, L. and Goodman, T., Science 1989. 243: 1716.
- 2 Guy-Grand, D., Cerf-Bensussan, N., Malissen, B., Malassis-Seris, M., Briottet, C. and Vassalli, P., J. Exp. Med. 1991. 173: 471.
- 3 Klein, J. R., Immunol. Today 1995. 16: 322.
- 4 Mosley, R. L., Styre, D. and Klein, J. R., J. Immunol. 1990. 145: 1369.
- 5 Lefrançois, L., LeCorre, R., Mayo, J., Bluestone, J. A. and Goodman, T., Cell 1990. 63: 333.
- 6 Guy-Grand, D. and Vassalli, P., Curr. Opin. Immunol. 1993. 5: 247.
- 7 He, Y.-W. and Malek, T. R., J. Exp. Med. 1996. 184: 289.
- 8 Maki, K., Sunaga, S., Komagane, Y., Kodaira, Y., Mabuchi, A., Karasuyama, H., Yokomura, K., Miyazaki, J. and Ikuta, K., Proc. Natl. Acad. Sci. USA 1996. 93: 7172.
- 9 Cao, X., Shores, E. W., Hu-Li, J., Anver, M. R., Kelsall, B. L., Russell, S. M., Drago, J., Noguchi, M., Grinberg, A., Bloom, E. T., William, E. P., Katz, S. I., Love, P. D. and Leonard, W. J., Immunity 1995. 2: 223.
- 10 Puddington, L., Olson, S. and Lefrançois, L., Immunity 1994. 1: 733.
- 11 Grabstein, K. H., Eisenman, J., Shanebeck, K., Rauch, C., Srinivasen, S., Fung, V., Beers, C., Richardson, J., Schoenborn, M. A., Ahdieh, M., Johnson, L., Alderson, M. R., Watson, J. D., Anderson, D. M. and Giri, J. G., Science 1994. 264: 965.
- 12 Giri, J. G., Ahdieh, M., Eisenman, J., Grabstein, K., Kumaki, S., Namen, A., Park, L. S., Cosmen, D. and Anderson, D., EMBO J. 1994. 13: 2822.
- 13 Tagaya, Y., Bamford, R. N., DeFilippis, A. P. and Waldmann, T. A., Immunity 1996. 4: 329.
- 14 Carson, W. E., Giri, J. G., Lindemann, M. L., Linett, M. L., Ahdieh, M., Paxton, R., Anderson, D., Eisenmann, J., Grabstein, K. and Caligiuri, M. A., J. Exp. Med. 1994. 180: 1395.
- 15 Armitage, R. J., Macduff, B. M., Eisenmann, J., Paxton, R. and Grabstein, K. H., J. Immunol. 1995. 154: 483.
- 16 Nishimura, H., Hiromatsu, K., Kobayashi, N., Grabstein, K. H., Paxton, R., Sugamura, K., Bluestone, J. A. and Yoshikai, Y., J. Immunol. 1996. 156: 663.
- 17 Giri, J. G., Anderson, D. M., Kumaki, S., Park, L. S., Grabstein, K. H. and Cosman, D. J., Leukocyte Biol. 1995. 57: 763.
- 18 Giri, J. G., Kumaki, S., Ahdieh, M., Friend, D. J., Loomis, A., Shanebeck, K., DuBosse, R., Cosman, D., Park, L. S. and Anderson, D. M., EMBO J. 1995. 14: 3654.
- 19 Di Santo, J. P., Kühn, R. and Müller, W., Immunol. Rev. 1995. 148: 19.
- 20 Quinn, L. S., Haugk, K. L. and Grabstein, K. H., Endocrinology 1995. 136: 3669.
- 21 Reinecker, H.-C., MacDermott, R. P., Mirau, S., Dignass, A. and Podolsky, D. K., Gastroenterology 1996. 111: 1706.
- 22 Di Santo, J. P., Müller, W., Guy-Grand, D., Fischer, A. and Rajewsky, K., Proc. Natl. Acad. Sci. USA 1995. 92: 377.
- 23 Leclercq, G., Debacker, V., Smedt, M. D. and Plum, J., J. Exp. Med. 1996. 184: 325.
- 24 Korsmeyer, S. J., Immunol. Today 1992. 13: 285.
- 25 Inagaki-Ohara, K., Kobayashi, N., Nishimura, H., Sakai, T., Matsumoto, Y., Hiromatsu, K., Awaya, A. and Yoshikai, Y., Cell Immunol. 1996. 171: 30.
- 26 Komano, H., Fujiura, M., Kawaguchi, M., Matsumoto, S., Hashimoto, Y., Obana, S., Mombaerts, P., Tonegawa, S., Yamamoto, H., Itohara, S., Nanno, M. and Ishikawa, H., Proc. Natl. Acad. Sci. USA 1995. 92: 6147.
- 27 Illera, V. A., Perandones, C. E., Stunz, L. L., Mower, D. A. Jr. and Ashman, R. F., J. Immunol. 1993. 151: 2965.
- 28 Atsuta, N., Nishimura, H., Nakamura, N., Emoto, M., Iwatsuki, T. and Yoshikai, Y., J. Immunol. 1995. 154: 676.
- 29 Yoshikai, Y., Reis, M. D. and Mak, T. W., Nature 1986. 324: 482.
- 30 Emoto, M., Danbara, H. and Yoshikai, Y., J. Exp. Med. 1992. 176: 363.
- 31 Von Freeden-Jeffry, U., Vieira, P., Lucian, L. A., McNeil, T., Burdach, S. E. G. and Murray, R., J. Exp. Med. 1995. 181: 1519.
- 32
Pereira, P.,
Gerber, D.,
Huang, S. Y. and
Tonegawa, S.,
J. Exp. Med.
1995.
182: 1921.
10.1084/jem.182.6.1921 Google Scholar
- 33 Bonneville, M., Janeway, C. A. Jr., Ito, K., Haser, W., Ishida, I., Nakanishi, N. and Tonegawa, S., Nature 1988. 336: 479.
- 34 Takagaki, Y., Decloux, A., Bonneville, M., Tonegawa, S., Nature 1989. 339: 712.
- 35 Viney, J. L. and MacDonald, T. T., Eur. J. Immunol. 1990. 20: 2809.
- 36 Cohen, J. J., Immunol. Today 1993. 14: 126.
- 37 Akbar, A. N., Borthwick, N. J., Wickremasinghe, R. G., Panayiotidis, P., Pilling, D., Bofill, M., Krajewski, S., Reed, J. C. and Salmon, M., Eur. J. Immunol. 1996. 26: 294.
- 38 Miyazaki, T., Liu, Z.-J., Kawahara, A., Minami, Y., Yamada, K., Tsujimoto, Y., Barsoumian, E. L., Perlmutter, R. M. and Taniguchi, T., Cell 1995. 81: 223.
- 39 Haas, W., Pereira, P. and Tonegawa, S., Annu. Rev. Immunol. 1993. 11: 637.
- 40 Park, S. Y., Saijo, K., Takahashi, T., Osawa, M., Arase, H., Hirayama, N., Miyake, K., Nakauchi, H., Shirasawa, T. and Saito, T., Immunity 1995. 3: 771.
- 41 Peschon, J. J., Morrissey, P. J., Grabstein, K. H., Ramsdell, F. J., Maraskovsky, E., Gliniak, B. C., Park, L. S., Ziegler, S. F., Williams, D. E., Ware, C. B., Meyer, J. D. and Davison, B. L., J. Exp. Med. 1994. 180: 1955.
- 42 Suzuki, H., Duncan, G. S., Takimoto, H. and Mak, T. W., J. Exp. Med. 1997. 185: 499.
- 43 Sadlack, B., Merz, H., Schorle, H., Schimpl, A., Feller, A. C. and Horak, I., Cell 1993. 75: 253.
- 44 Havran, W. L. and Aliison, J. P., Nature 1988. 335: 443.
- 45 Okazaki, H., Ito, M., Sudo, T., Hattori, M., Kano, S., Katsura, Y. and Minato, N., J. Immunol. 1989. 143: 2917.
- 46 Watoson, J. D., Morrissey, P. J., Namen, A. E., Conlon, P. J. and Widmer, M. B., J. Immunol. 1989. 143: 1215.
- 47 Watanabe, Y., Sudo, T., Minato, N., Ohnishi, A. and Katsura, Y., Int. Immunol. 1991. 3: 1067.
- 48 Appasamy, P. M., J. Immunol. 1992. 149: 1649.
- 49 Uehira, M., Matsuda, H., Hikita, I., Sakata, T., Fujiwara, H. and Nishimoto, H., Int. Immunol. 1993. 5: 1619.
- 50 Fujihashi, K., Kawabata, S., Hiroi, T., Yamamoto, M., McGhee, J. R., Nishikawa, S. and Kiyono, H., Proc. Natl. Acad. Sci. USA 1996. 93: 3613.
- 51 Appasamy, P. M., Kenniston, T. W. Jr., Weng, Y., Holt, E. C., Kost, J. and Chambers, W. H., J. Exp. Med. 1993. 178: 2201.
- 52 Murosaki, S., Inagaki-Ohara, K., Kusaka, H., Ikeda, H. and Yoshikai, Y., Microbiol. Immunol. 1997. 41: 139.
Citing Literature
