Recombinant human tumor necrosis factor (rhuTNF) induced DNA fragmentation in sensitive cell lines. This fragmentation was similar to that caused by lymphotoxincontaining cytotoxic T cell culture supernatants. The percentage of DNA cleaved correlated with the degree of cell growth inhibition shown by individual cell lines. DNA fragmentation was first seen after 12 h treatment, and increased slowly with time. The presence of 100 μM ZnSO₄ inhibited the rhuTNF-induced DNA cleavage in MCF-7 cells. Recombinant interferon-γ (rhuIFN-γ) did not induce DNA cleavage, although it reduced the growth of all the cell lines used in this study. However, it interacted with rhuTNF to produce a doubling in the percentage of DNA fragmentation, and increased cytotoxicity in rhuTNF-sensitive cell lines. Pretreatment with rhuIFN-γ for 1 h prior to rhuTNF treatment also enhanced DNA fragmentation and cell killing.

References

1 Beutler, B. and Cerami, A., Nature 1986. 320: 584.
10.1038/320584a0
CAS PubMed Web of Science® Google Scholar
2 Carswell, E. A., Old, L. J., Kassell, R. L., Green, S., Fiore, N. and Williamson, B., Proc. Natl. Acad. Sci. USA 1975. 72: 366.
10.1073/pnas.72.9.3666
Web of Science® Google Scholar
3 Marmenout, A., Fransen, L., Tavernier, J., Van der Heyden, J., Tizard, R., Kawashima, E., Shaw, A., Johnson, K.-J., Semon, D., Muller, R., Rysschaert, M.-R., Van Vliet, A. and Fiers, W., Eur. J. Biochem. 1985. 152: 515.
10.1111/j.1432-1033.1985.tb09226.x
CAS PubMed Web of Science® Google Scholar
4 Wang, A. M., Creasy, A. A., Ladner, M. B., Lin, L. S., Strickler, J., Van Arsdell, J. N., Yamamoto, R. and Mark, D. F., Science 1985. 228: 149.
10.1126/science.3856324
CAS PubMed Web of Science® Google Scholar
5 Pennica, D., Hayflick, J. S., Bringman, T. S., Palladino, M. A. and Goeddel, D. V., Proc. Natl. Acad. Sci. USA 1985. 82: 6060.
10.1073/pnas.82.18.6060
CAS PubMed Web of Science® Google Scholar
6 Philip, R. and Epstein, L. B., Nature 1986. 323: 86.
10.1038/323086a0
CAS PubMed Web of Science® Google Scholar
7 Urban, J. L., Shepard, H. M., Rothstein, L. J., Sugarman, B. J. and Schreiber, H., Proc. Natl. Acad. Sci. USA 1986. 83: 5233.
10.1073/pnas.83.14.5233
CAS PubMed Web of Science® Google Scholar
8 Bersani, L., Colotta, F. and Mantovani, A., Immunology 1986. 59: 523.
Google Scholar
9 Sugarman, B. J., Aggarwal, B. B., Hass, P. E., Figari, I. S., Palladino, M. A. and Shepard, H. M., Science 1985. 230: 943.
10.1126/science.3933111
CAS PubMed Web of Science® Google Scholar
10 Fransen, L., Van der Heyden, J., Ruysschaert, M. R. and Fiers, W., Eur. J. Cancer Clin. Onc. 1986. 22: 419.
10.1016/0277-5379(86)90107-0
CAS PubMed Web of Science® Google Scholar
11 Balkwill, F. R., Lee, A., Aldam, G., Moodie, E., Thomas, J. A., Tavernier, J. and Fiers, W., Cancer Res. 1986. 46: 3990.
CAS PubMed Web of Science® Google Scholar
12 Daeltry, G. B., Naylor, M. S., Fiers, W. and Balkwill, F. R., Exp. Cell Res. 1986, in press.
Google Scholar
13 Fiers, W., Brouckaert, P., Guisez, Y., Remaut, F., Van Roy, F., Deros, R., Fransen, L., Leroux-Roels, G., Marmenout, A., Tavernier, J. and Van der Heyden, J., in W. E. Steward and H. Schellekins (Eds.), The Biology of the Interferon System, Elsevier, Amsterdam 1985, p. 241.
Google Scholar
14 Vilcek, J., Palommbella, V. J., Henriksen-Destefano, D., Swenson, C., Feinman, R., Hirai, M. and Tsujimoto, K., J. Exp. Med. 1986. 163: 632.
10.1084/jem.163.3.632
CAS PubMed Web of Science® Google Scholar
15 Tsujimoto, M., Yip, Y. K. and Vilcek, J., Proc. Natl. Acad. Sci. USA 1985. 82: 7626.
10.1073/pnas.82.22.7626
CAS PubMed Web of Science® Google Scholar
16 Tsujimoto, M., Yik, Y. K. and Vilcek, J., J. Immunol. 1986. 136: 2441.
10.4049/jimmunol.136.7.2441
CAS PubMed Web of Science® Google Scholar
17 Schmid, D. S., Tite, J. P. and Ruddle, N. H., Proc. Natl. Acad. Sci. USA 1986. 83: 1881.
10.1073/pnas.83.6.1881
CAS PubMed Web of Science® Google Scholar
18 Balkwill, F. R., Goldstein, L. and Stebbing, N., Int. J. Cancer 1985. 35: 613.
10.1002/ijc.2910350508
CAS PubMed Web of Science® Google Scholar
19 Lasfargues, E. Y. and Ozello, L., J. Natl. Cancer Inst. 1958. 21: 1131.
CAS PubMed Web of Science® Google Scholar
20 Soule, H. D., Cozquez, J., Long, A., Albert, S. and Brennan, J. Natl. Cancer Inst. 1973. 51: 1409.
10.1093/jnci/51.5.1409
PubMed Web of Science® Google Scholar
21 Fogh, J. and Trempe, G., Human Tumor Cells in vitro, Plenum Press, New York 1975, p. 115.
10.1007/978-1-4757-1647-4_5
Google Scholar
22 Duke, R. C., Chervenak, R. and Cohen, J. J., Proc. Natl. Acad. Sci. USA 1983. 80: 6361.
10.1073/pnas.80.20.6361
CAS PubMed Web of Science® Google Scholar
23 Balkwill, F. R. and Bokhon'ko, A., Exp. Cell. Res. 1984. 155: 190.
10.1016/0014-4827(84)90780-8
CAS PubMed Web of Science® Google Scholar
24 Wyllie, A. H., Kerr, J. F. R. and Currie, A. R., Int. Rev. Cytol. 1980. 68: 251.
10.1016/S0074-7696(08)62312-8
CAS PubMed Google Scholar
25 Aggarwal, B. B., Essaln, T. E. and Hass, P. E., Nature 1985. 318: 665.
10.1038/318665a0
CAS PubMed Web of Science® Google Scholar
26 Friedkin, M. and Rozengurt, E., Adv. Enzyme Regul. 1981. 19: 39.
10.1016/0065-2571(81)90008-X
CAS Google Scholar
27 Aggarwal, B. B., Essalu, T. E. and Hass, P. E., Nature 1985. 318: 665.
10.1038/318665a0
CAS PubMed Web of Science® Google Scholar
28 Tsujimoto, M., Feinman, R. and Vilcek, J., J. Immunol. 1986. 137: 2272.
10.4049/jimmunol.137.7.2272
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume17, Issue5

1987

Pages 689-693

DNA fragmentation and cytotoxicity caused by tumor necrosis factor is enhanced by interferon-γ

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

DNA fragmentation and cytotoxicity caused by tumor necrosis factor is enhanced by interferon-γ

Abstract

References

Citing Literature

References

Related

Information