Detection of high-risk human papillomavirus (HPV) DNA by Hybrid Capture II in women referred due to atypical glandular cells in the primary screening
Eliane R.Z.M. Oliveira
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Search for more papers by this authorCorresponding Author
Sophie F.M. Derchain
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Rua Antônio Hossri, 629 - Cidade Universitária, 13083-370 - Campinas - SP, BrazilSearch for more papers by this authorSilvia H. Rabelo-Santos
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Faculty of Pharmacy, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil
Search for more papers by this authorMaria Cristina A. Westin
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Search for more papers by this authorLuiz Carlos Zeferino
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Search for more papers by this authorElisabete A. Campos
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Search for more papers by this authorKari J. Syrjanen
Cytopathology Unit, Laboratory of Epidemiology, National Institute of Health (ISS), Rome, Italy
Search for more papers by this authorEliane R.Z.M. Oliveira
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Search for more papers by this authorCorresponding Author
Sophie F.M. Derchain
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Rua Antônio Hossri, 629 - Cidade Universitária, 13083-370 - Campinas - SP, BrazilSearch for more papers by this authorSilvia H. Rabelo-Santos
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Faculty of Pharmacy, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil
Search for more papers by this authorMaria Cristina A. Westin
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Search for more papers by this authorLuiz Carlos Zeferino
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Search for more papers by this authorElisabete A. Campos
Department of Obstetrics and Gynecology Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
Search for more papers by this authorKari J. Syrjanen
Cytopathology Unit, Laboratory of Epidemiology, National Institute of Health (ISS), Rome, Italy
Search for more papers by this authorAbstract
We assessed the detection of high-risk human papillomavirus DNA (HPV-DNA) in women examined by a second Pap smear due to atypical glandular cells (AGC) detected in their screening Pap smear. In 91 women included in the study, a second Pap smear was taken and HPV-DNA test was performed using Hybrid Capture II (HC II). The second Pap smear showed no abnormalities in 28 (31%) cases, ACG in 17 (19%) cases, and high-grade squamous intraepithelial lesions (HSIL) or worse in 24 (26%). HC II test was positive in 36% of the cases. Considering the second Pap smear diagnosis, HPV-DNA was detected in 87% of the women with HSIL, 100% of women with in situ adenocarcinoma, and only in 11% of the women with no abnormalities. The use of the second Pap smear combined with HPV-DNA may improve the management of women with AGC in the primary screening. Diagn. Cytopathol. 2004;31:19–22. © 2004 Wiley-Liss, Inc.
References
- 1
Kurman RJ,
Soloman D.
The Bethesda System for reporting cervical/vaginal diagnoses.
Bethesda MD,
Springer,
1994.
10.1007/978-1-4684-0201-8 Google Scholar
- 2 Raab SS, Snider TE, Potts, SA, et al. Atypical glandular cells of undetermined significance. Diagnostic accuracy and iterobserver variability using select cytologic criteria. Am J Clin Pathol 1997; 107: 299–307.
- 3 Burja IT, Thompson SK, Sawyer WI, Shurbaji MS, Atypical glandular cells of undetermined significance on cervical smears. A study with cytohistologic correlation. Acta Cytol 1999; 43: 351–356.
- 4 Raab SS. Can glandular lesions be diagnosed in Pap smear cytology?. Diagn Cytopathol 2000; 23: 127–133.
- 5 Chieng DC, Elgert PA, Cangiarella JF, Cohen JM. Clinical significance of atypical glandular cells of undetermined significance. A follow-up study from an academic medical center. Acta Cytol 2000a; 44: 557–566.
- 6 Chieng DC, Elgert PA, Cangiarella JF, Cohen JM. Variation in the incidence of AGUS between different patient populations. Acta Cytol 2000b; 45: 287–299.
- 7 DeMay RM. The Pap smear. In: The art and the science of cytopathology. Chicago: ASCP Press; 1993, p 61–185.
- 8 Lee KR, Manna EA, Jones MA. Comparative cytologic features of adenocarcinoma in situ of uterine cervix. Acta Cytol 1991; 35: 117–126.
- 9
Biscotti, CV,
Gero, MA,
Toddy, SM,
Fischier, DF,
Easley, KA.
Endocervical adenocarcinoma in situ: an analyses of cellular features.
Diagn Cytopathol
1997;
17:
326–332.
10.1002/(SICI)1097-0339(199711)17:5<326::AID-DC4>3.0.CO;2-8 CAS PubMed Web of Science® Google Scholar
- 10 Solomon D, Davey DD, Kurman RJ, et al. The 2001 Bethesda System—terminology for reporting results of cervical cytology. JAMA 2002; 287: 2114–2119.
- 11 Anciaux D, Lawrence WD, Gregoire L. Glandular lesions of the uterine cervix: prognostic implications of human papillomavirus status. Int J Gynecol Pathol 1997; 16: 103–110.
- 12 Hörding U, Daugaard S, Visfeldt J. Adenocarcinomas of the cervix and adenocarcinoma of the endometrium: distintion with PCR-mediated detection of HPV-DNA. APMIS 1997; 105: 313–316.
- 13 Andersson S, Rylander E, Larsson B, et al.. The role of human papillomavirus in cervical adenocarcinoma carcinogenesis. Eur J Cancer 2001; 37: 246–250.
- 14 Ronnet BM, Manos MM, Ransley JE, et al. Atypical glandular cells of undetermined significance: cytopathologic features, histopathologic results, and human papillomavirus DNA detection. Hum Pathol 1999; 30: 816–825.
- 15
Walboomers JM,
Jacobs MV,
Manos MM, et al..
Human papillomavirus is a necessary cause of invasive cervical cancer worldwide.
J Pathol
1999;
189:
12–19.
10.1002/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F CAS PubMed Web of Science® Google Scholar
- 16 National Cancer Institute Workshop. Bethesda System 2001 available at http.www.bethesda2001.cancer.gov
- 17 Wilbur DC. Endocervical glandular atypia: a “new” problem for the cytopathologist. Diagn Cytopathol 1995; 13: 463–469.
- 18 Raab SS.; Geisinger KR, Silverman JF, Thomas PA, Stanley MW. Interobserver variability of a Papanicolaou smear diagnosis of atypical glandular cells of undetermined significance. Am J Clin Pathol 1998; 110: 653–659.
- 19 Diaz-Rosario LA, Kabawat SE. Cell block preparation by inverted filter sedimentation is useful in the differential diagnosis of atypical glandular cells of undetermined significance in ThinPrep specimens. Cancer 2000; 90: 265–272.
- 20 Soofer SB, Sidawy MK. Atypical glandular cells of undetermined significance: clinically significant lesions and means of patient follow-up. Cancer 2000; 90: 207–214.
- 21 Geier CS.; Wilson M.; Creasman W. Cinical evaluation of atypical glandular cells of undetermined significance. Am J Obstet Gynecol 2001; 184: 64–69.
- 22 Lee KR, Manna EA, St Jonh T. Atypical endocervical glandular cells: accuracy of cytologic diagnosis. Diagn Cytopathol 1995; 13: 202–208.
- 23 Raab SS.; Bishop NS, Zaleski MS. Effect of cervical disease history on outcomes of women who have a Pap diagnosis of atypical glandular cells of undetermined significance. Gynecol Oncol 1999; 74: 460–464.
- 24 Wilbr D. Cytology of endocervix, endometrium, and upper female genital tract. In: TA Bonfiglio, YS Erozan, editors. Gynecologic cytopathology: Philadelphia: Lippincott-Raven; 1997. p 107–169.
- 25 Solomon D, Frable WJ, Vooijs GP, et al. ASCUS and AGUS criteria. International Academy of Cytology Task Force Summary. Diagnostic cytology towards the 21st century: an international expert conference and tutorial. Acta Cytol 1998; 42: 16–24.
Citing Literature
