Stereoselective Synthesis and Immunological Evaluation of Common O-antigen of P. mirabilis OE and P. vulgaris TG103
Xiaopei Wu
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
‡These authors contributed equally.
Search for more papers by this authorJinsheng Lai
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
‡These authors contributed equally.
Search for more papers by this authorKexin Yang
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, and Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122 China
‡These authors contributed equally.
Search for more papers by this authorYunxia Xue
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
Search for more papers by this authorJinxi Liao
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
Search for more papers by this authorLiming Wang
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
Search for more papers by this authorJian-song Sun
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, and Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122 China
Search for more papers by this authorCorresponding Author
Jing Hu
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, and Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122 China
E-mail: [email protected]; [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Jian Yin
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, and Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122 China
E-mail: [email protected]; [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Qingju Zhang
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
E-mail: [email protected]; [email protected]; [email protected]Search for more papers by this authorXiaopei Wu
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
‡These authors contributed equally.
Search for more papers by this authorJinsheng Lai
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
‡These authors contributed equally.
Search for more papers by this authorKexin Yang
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, and Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122 China
‡These authors contributed equally.
Search for more papers by this authorYunxia Xue
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
Search for more papers by this authorJinxi Liao
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
Search for more papers by this authorLiming Wang
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
Search for more papers by this authorJian-song Sun
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, and Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122 China
Search for more papers by this authorCorresponding Author
Jing Hu
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, and Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122 China
E-mail: [email protected]; [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Jian Yin
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, and Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, 214122 China
E-mail: [email protected]; [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Qingju Zhang
National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022 China
E-mail: [email protected]; [email protected]; [email protected]Search for more papers by this authorComprehensive Summary
Proteus species especially Proteus mirabilis and Proteus vulgaris are zoonotic pathogens which can cause public health disease. Owing to their antibiotic-resistance, developing vaccines against these pathogens is urgently required. Herein, we describe the first synthesis of the common O-antigen of Proteus mirabilis OE and Proteus vulgaris TG 103. The repeating unit incorporates two challenging 1,2-cis-glycosidic bonds: the 1,2-cis-2-acetamido-2-deoxy-glucosidic bonds were successfully constructed by use of conformation-restrained 2-nitroglucal donor under bifunctional organothiourea catalysis; while the 1,2-cis-2-acetamido-2-deoxy-galactosidic bonds were formed by use of di-tert-butylsilylidene protected 2-azidogalactose donors. The synthetic fragments were screened by glycan microarray with immunized rabbit sera against purified LPS from Proteus mirabilis O54. The results revealed that the synthetic common O-antigens both hexasaccharide 2 and nonasaccharide 3 can strongly bind with the IgG antibodies (Abs) in the sera, which is highly valuable for further immunological investigation in synthetic carbohydrate-based vaccine development.
Supporting Information
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| cjoc202200638-sup-0001-Supinfo.pdfPDF document, 14.5 MB |
Appendix S1: Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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