A New Nanogold-Labeled Immunoresonance Scattering Spectral Probe for Determination of Trace IgG
Zhi-Liang Jiang
School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China
Department of Material and Chemical Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China
Search for more papers by this authorYan Li
School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorShuang-Jiao Sun
Department of Material and Chemical Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China
Search for more papers by this authorBing Chen
School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorZhi-Liang Jiang
School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China
Department of Material and Chemical Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China
Search for more papers by this authorYan Li
School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorShuang-Jiao Sun
Department of Material and Chemical Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China
Search for more papers by this authorBing Chen
School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorAbstract
A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti-human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold-labeled goat anti-human IgG and IgG took place, and the resonance scattering intensity at 580 nm (I580 nm) was enhanced greatly. The enhanced intensity ΔIRS is proportional to the IgG concentration from 1.3 to 1.5×103 ng·mL−1, with a detection limit of 0.78 ng·mL−1. This assay showed high sensitivity and good selectivity for quantitative determination of IgG in human serum, with satisfactory results.
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