Physico-chemical Properties and Bioactivities of a Glycoconjugate LbGp5B from Lycium barbarum L

A glycoconjugate designated as LbGp5B was isolated from the fruit of Lycium barbarian L. and purified to homogeneity by gel filtration. LbGpSB is composed of rhamnose (Una), arabinose (Ara), galactose (Gal), glucose (Glc), galacturonic add (GalA) and seventeen amino adds. The molecular weight of LbGpSB was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and by matrix-assisted laser desorption/ionization (MALDI) time of fight (TOF) mass spectrometry (MS). The preliminary experiments showed that LbGpSB promoted splenocyte proliferation in mice and inhibited the peroxidation of low density lipoprotein (LDL).