A New Method to Measure Electroosmotic Flow Mobility of Capillary Electrophoresis by Abrupt Change of Current De-noising via Wavelet Transform

The electroosmotic flow mobility has been measured by the combination of monitoring the change in electric current during electrophoretic run and operating the wavelet transform. Once the sample solvent zone with different ionic strength from background electrolyte migrated from the capillary, a sudden change in current could be observed from the electric current record of time history. The exact time (in the middle of abrupt range) corresponding to the abrupt change in current was determined by wavelet transform. This work showed that the electroosmotic flow mobility determined by the proposed method was in a good agreement with the neutral marker method commonly used.