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The principle of optimization of binary mobile phase composition of multi-step linear gradient elution

Corresponding Author

Han-Fa Zou

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, DalianSearch for more papers by this author

Yu-Kui Zhang,

Yu-Kui Zhang

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

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Li-Fu Dong,

Li-Fu Dong

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

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Mian-Sheng Bao,

Mian-Sheng Bao

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

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Pei-Zhang Lu,

Pei-Zhang Lu

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

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Han-Fa Zou,

Corresponding Author

Han-Fa Zou

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, DalianSearch for more papers by this author

Yu-Kui Zhang,

Yu-Kui Zhang

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

Search for more papers by this author

Li-Fu Dong,

Li-Fu Dong

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

Search for more papers by this author

Mian-Sheng Bao,

Mian-Sheng Bao

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

Search for more papers by this author

Pei-Zhang Lu,

Pei-Zhang Lu

Dalian Institute of Chemical Physics, Academia Sinica, Dalian Chromatographic R&D Center of China, Dalian

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First published: December 1989

https://doi.org/10.1002/cjoc.19890070606

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Abstract

The basic principle of optimal method called “moving overlapping resolution mapping method” to select the optimal binary mobile phase composition of multi-step linear gradient liquid chromatography is discussed with simultaneously considering effects of position of solute inside the column and mobile phase composition on peak resolution and retention value, then a BASIC program based on this principle is developed in IBM-PC computer. The validities of both principle of optimization and BASIC program are confirmed by separation of samples containing bile acids and PAHs in RP-HPLC.

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Volume7, Issue6

December 1989

Pages 511-519

The principle of optimization of binary mobile phase composition of multi-step linear gradient elution

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The principle of optimization of binary mobile phase composition of multi-step linear gradient elution

Abstract

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