Research Article

Preparation of Polyglycidylmethacrylate Macropore Beads and Application in Candida species 99–125 Lipase Immobilization

R. Zhao

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

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J. Lu,

J. Lu

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

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T. Tan,

Corresponding Author

T. Tan

[email protected]

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, ChinaSearch for more papers by this author

R. Zhao,

R. Zhao

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

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J. Lu,

J. Lu

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

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T. Tan,

Corresponding Author

T. Tan

[email protected]

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, ChinaSearch for more papers by this author

First published: 26 November 2010

https://doi.org/10.1002/ceat.201000263

Citations: 14

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Abstract

Polyglycidylmethacrylate beads with macroporous structure were synthesized by suspension polymerization and directly used for Candida species 99–125 lipase immobilization because of their rich surface epoxy groups. The properties of the synthesized polymer beads were characterized by scanning electron microscopy and FT-IR spectroscopy. To increase the enzyme activity, divinyl benzene and toluene/heptane were used as cross-linker and porogen, respectively, and the effects of cross-linker content and porogen content on lipase immobilization were studied. After optimizing the synthesis conditions, the optimum lipase concentration for immobilization was determined as 10 mg mL^–1. The immobilized lipase showed a broader pH stability and higher temperature stability. The immobilized enzyme could be reused for ten batches with 62 % of residual activity.

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Volume34, Issue1

January, 2011

Pages 93-97

Preparation of Polyglycidylmethacrylate Macropore Beads and Application in Candida species 99–125 Lipase Immobilization

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Preparation of Polyglycidylmethacrylate Macropore Beads and Application in Candida species 99–125 Lipase Immobilization

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