Inhibition of Alzheimer's Aβ1-42 Fibrillogenesis and Removal of Copper Ions by Polypeptides Modified Gold Nanoparticles
Binbin Zhou
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorXingxin Sheng
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorHao Xie
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorSisi Zhou
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorCorresponding Author
Ming Zhong
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorCorresponding Author
An Liu
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorBinbin Zhou
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorXingxin Sheng
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorHao Xie
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorSisi Zhou
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorCorresponding Author
Ming Zhong
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorCorresponding Author
An Liu
College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan, 414006 China
Search for more papers by this authorAbstract
Aggregation and fibrillation of β-amyloid peptides (Aβ) as well as accumulation of toxic metal ions have been believed to be the central events to cause Alzheimer's disease (AD). Thus, an attractive therapeutic tactic for AD is to design and synthesize inhibitors and metal chelators to prevent Aβ aggregation and chelate toxic metal ions. In this study, the polypeptide functionalized gold nanoparticles (PFGNP) were obtained by modifying polypeptides Cys-Gly-Gly-Gly-Leu-Pro-Phe-Phe-Asp (CGGGLPFFD) and Cys-Gly-Gly-Gly-Gly-Gly-His (CGGGGGH) onto gold nanoparticles through gold-sulfur bond. The inhibitory properties of PFGNP toward Aβ1-42 fibril formation was assessed by thioflavin T (ThT) fluorescence method and corroborated by atomic force microscopy analysis. The ability of PFGNP to complex copper ions was studied by electrochemical method. The experimental results reveal that PFGNP can effectively chelate copper ions and significantly inhibit the fibrillation of Aβ1-42. Moreover, PFGNP exhibits significantly protective effect on Aβ-induced cytotoxicity toward human neuroblastoma SH-SY5Y cells.
Graphical Abstract
Conflict of interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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