MMPREDOX/NO Interplay in Periodontitis and Its Inhibition with Satureja hortensis L. Essential Oil
Corresponding Author
Fares Zeidán-Chuliá
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, ICBS-UFRGS. Rua Ramiro Barcelos 2600 – ANEXO, Porto Alegre, 90035-003, RS, Brasil, (phone: +55-51-33085577; fax: +55-51-33085535)
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, ICBS-UFRGS. Rua Ramiro Barcelos 2600 – ANEXO, Porto Alegre, 90035-003, RS, Brasil, (phone: +55-51-33085577; fax: +55-51-33085535)Search for more papers by this authorBen-Hur Neves de Oliveira
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, ICBS-UFRGS. Rua Ramiro Barcelos 2600 – ANEXO, Porto Alegre, 90035-003, RS, Brasil, (phone: +55-51-33085577; fax: +55-51-33085535)
Search for more papers by this authorMervi Gursoy
Institute of Dentistry, University of Turku, FIN-Turku
Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), FIN-Helsinki
Search for more papers by this authorEija Könönen
Institute of Dentistry, University of Turku, FIN-Turku
Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), FIN-Helsinki
Search for more papers by this authorJosé Cláudio Fonseca Moreira
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, ICBS-UFRGS. Rua Ramiro Barcelos 2600 – ANEXO, Porto Alegre, 90035-003, RS, Brasil, (phone: +55-51-33085577; fax: +55-51-33085535)
These authors jointly directed this work.
Search for more papers by this authorUlvi K. Gursoy
Institute of Dentistry, University of Turku, FIN-Turku
Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), FIN-Helsinki
These authors jointly directed this work.
Search for more papers by this authorVeli-Jukka Uitto
Institute of Dentistry and Department of Oral and Maxillofacial Surgery, Helsinki University Central Hospital, University of Helsinki, FIN-Helsinki
These authors jointly directed this work.
Search for more papers by this authorCorresponding Author
Fares Zeidán-Chuliá
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, ICBS-UFRGS. Rua Ramiro Barcelos 2600 – ANEXO, Porto Alegre, 90035-003, RS, Brasil, (phone: +55-51-33085577; fax: +55-51-33085535)
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, ICBS-UFRGS. Rua Ramiro Barcelos 2600 – ANEXO, Porto Alegre, 90035-003, RS, Brasil, (phone: +55-51-33085577; fax: +55-51-33085535)Search for more papers by this authorBen-Hur Neves de Oliveira
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, ICBS-UFRGS. Rua Ramiro Barcelos 2600 – ANEXO, Porto Alegre, 90035-003, RS, Brasil, (phone: +55-51-33085577; fax: +55-51-33085535)
Search for more papers by this authorMervi Gursoy
Institute of Dentistry, University of Turku, FIN-Turku
Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), FIN-Helsinki
Search for more papers by this authorEija Könönen
Institute of Dentistry, University of Turku, FIN-Turku
Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), FIN-Helsinki
Search for more papers by this authorJosé Cláudio Fonseca Moreira
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, ICBS-UFRGS. Rua Ramiro Barcelos 2600 – ANEXO, Porto Alegre, 90035-003, RS, Brasil, (phone: +55-51-33085577; fax: +55-51-33085535)
These authors jointly directed this work.
Search for more papers by this authorUlvi K. Gursoy
Institute of Dentistry, University of Turku, FIN-Turku
Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), FIN-Helsinki
These authors jointly directed this work.
Search for more papers by this authorVeli-Jukka Uitto
Institute of Dentistry and Department of Oral and Maxillofacial Surgery, Helsinki University Central Hospital, University of Helsinki, FIN-Helsinki
These authors jointly directed this work.
Search for more papers by this authorAbstract
Satureja hortensis L. is an aromatic plant with antibacterial and antibiofilm activities against periodontopathogens. Here, we attempted to find out whether the antioxidant properties of S. hortensis L. essential oil (EO) could be used to inhibit matrix metalloproteinase (MMP) activities and prevent the induction of cell death by a pro-oxidant insult. First, a landscape analysis of MMP and REDOX/nitric oxide (NO)-related genes was performed (MRN model), and array data from periodontitis patients were plotted over the newly developed model. Thereafter, the antigelatinolytic activity of S. hortensis L. EO and its preventive effect against hydrogen peroxide (H2O2)-induced cell death were tested in vitro (HaCaT cells). Up-regulation of MMP genes in the MRN network (except for MMP-10, -15, -16, -20, -25, and -26) and differential expression of genes coding for antioxidant enzymes were found among others in periodontitis samples. MMP2 and MMP9 were central genes in the MRN network model. Moreover, treatments with 1 and 5 μl/ml of S. hortensis L. EO inhibited both MMP-2 and MMP-9 activities, and H2O2-induced cell death in vitro. We concluded that S. hortensis L. EO could be a promising host-modulating agent, since oxidative stress and excessive MMP expression/activity are typical hallmarks of periodontal pathogenesis.
Graphical Abstract
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