Fluorescence line narrowing study of the ground-state and first-excited-state vibrational frequencies of Sn and Zn cytochromes c

Monique Laberge

Johnson Research Foundation, Department of Biochemistry & Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

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Jane M. Vanderkooi,

Jane M. Vanderkooi

Johnson Research Foundation, Department of Biochemistry & Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

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Monique Laberge,

Monique Laberge

Johnson Research Foundation, Department of Biochemistry & Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

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Jane M. Vanderkooi,

Jane M. Vanderkooi

Johnson Research Foundation, Department of Biochemistry & Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

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First published: 1995

https://doi.org/10.1002/bspy.350010606

Citations: 1

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Abstract

Fluorescence-line-narrowing (FLN) spectra are presented for Sn cytochrome c (Snc) obtained under Q_0-0 and Q_0-1 excitation and for Zn cytochrome c (Znc) under Q_0-1 excitation. Vibrational frequencies of the ground and first excited states are reported for Snc. Additional excited-state Znc frequencies are also presented, thus contributing to a previous FLN Znc investigation. The spectra of Snc shows more phonon broadening than for Znc, but vibrational frequencies of the S₀ and S₁ states could be obtained and the distribution function of the 0, 0 transition was determined. Snc and Znc showed good agreement in their excited-state vibrational frequencies. Some S₁ vibrational frequencies of Znc are seen to downshift with respect to ground-state vibrations, suggesting that the porphyrin S₀ to S₁ expansion observed in other porphyrin systems also occurs in cytochrome c derivatives. © 1995 John Wiley & Sons, Inc.

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Volume1, Issue6

1995

Pages 413-421

Fluorescence line narrowing study of the ground-state and first-excited-state vibrational frequencies of Sn and Zn cytochromes c

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Fluorescence line narrowing study of the ground-state and first-excited-state vibrational frequencies of Sn and Zn cytochromes c

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