Erratum
Free Access
Erratum: Combined effects of 60 Hz electromagnetic field exposure with various stress factors on cellular transformation in NIH3T3 cells
First published: 08 October 2013
In the original article [Lee et al., 2012], the published Figure 3b is an image of Soft agar assay, but the same picture was uploaded to represent both IR and IR-ELF exposures. We have attached a corrected version of Figure 3. The scientific conclusions of the article were not affected by this error in anyway. The authors regret the mistake.
Figure 1
Schematic of the 60 Hz ELF magnetic field exposure system for in vitro studies. a: System structure of the magnetic field generator. b: Sixty hertz magnetic field exposure system included a power supply, magnetic field generating coils and a ferrite shielding system in a CO2 incubator. c: ELF magnetic field distribution for the middle floor of the exposure system. d: Maintenance of temperature in the exposure location during the exposure period.
Figure 2
a: Anchorage-independent growth of NIH3T3 cells in soft agar after 4 h treatment of indicated concentrations of H2O2. b: Exposure to ionizing radiation (IR) with a single or fractionated dose. *Significantly different from untreated control cells at P < 0.05 (mean ± SD).
Figure 3
a,b: Number of colonies with 4 h exposure of 1 mT ELF with or without two doses of 1 Gy IR. c,d: Treatment with 100 µM H2O2. b,d: Typical morphology of colonies after IR exposure or H2O2 treatment is also shown (white arrows). Scale bar = 50 µm. *Significantly different from untreated control cells at P < 0.05 (mean ± SD). CON, unexposed control cells; ELF, ELF-exposed cells; IR, ionizing radiation-exposed cells; H2O2, H2O2-exposed cells; ELF + IR, combined exposure of ELF and IR; ELF + H2O2, combined exposure of ELF and H2O2.
Figure 4
a: Imunoblotting of c-Myc protein after transfection of empty vector (V5) or c-Myc was performed in NIH3T3 cells. b: Cellular transformation assay in control and V5 or c-Myc transfected NIH3T3 cells. c: Morphologyand d: number of colonies with exposure to 4 h ELF (1 mT), 2 Gy IR, or ELF + IR in c-Myc transfected and V5 transfected cells. Scale bar = 50 µm. *Significantly different from corresponding untreated control cells at P < 0.05 (mean ± SD). CON, unexposed control cells; V5, pcDNA-V5 control plasmid transfected cells; c-Myc, c-Myc-V5 plasmid transfected cells; ELF, ELF-exposed cells; IR, ionizing radiation-exposed cells; ELF + IR, combined exposure of ELF and IR.
