Low electromagnetic field (50 Hz) induces differentiation on primary human oral keratinocytes (HOK)
Vanessa Manni
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorAntonella Lisi
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorSabrina Rieti
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorAnnalucia Serafino
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorMario Ledda
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorLivio Giuliani
Istituto Superiore per la Prevenzione E la Sicurezza del Lavoro (ISPESL), Dipartimento Venezia, Venice, Italy
Istituto Superiore per la Prevenzione E la Sicurezza del Lavoro (ISPESL), Dipartimento Insediamenti Produttivi e Interazione con l'Ambiente (DIPIA), Rome, Italy
Search for more papers by this authorDonatella Sacco
Istituto Superiore per la Prevenzione E la Sicurezza del Lavoro (ISPESL), Dipartimento Insediamenti Produttivi e Interazione con l'Ambiente (DIPIA), Rome, Italy
Search for more papers by this authorEnrico D'Emilia
Istituto Superiore per la Prevenzione E la Sicurezza del Lavoro (ISPESL), Dipartimento Insediamenti Produttivi e Interazione con l'Ambiente (DIPIA), Rome, Italy
Search for more papers by this authorCorresponding Author
Settimio Grimaldi
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Istituto di Neurobiologia e Medicina Molecolare–Consiglio Nazionale delle Ricerche, Via Fosso del Cavaliere 100, 00133 Rome, Italy.Search for more papers by this authorVanessa Manni
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorAntonella Lisi
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorSabrina Rieti
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorAnnalucia Serafino
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorMario Ledda
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Search for more papers by this authorLivio Giuliani
Istituto Superiore per la Prevenzione E la Sicurezza del Lavoro (ISPESL), Dipartimento Venezia, Venice, Italy
Istituto Superiore per la Prevenzione E la Sicurezza del Lavoro (ISPESL), Dipartimento Insediamenti Produttivi e Interazione con l'Ambiente (DIPIA), Rome, Italy
Search for more papers by this authorDonatella Sacco
Istituto Superiore per la Prevenzione E la Sicurezza del Lavoro (ISPESL), Dipartimento Insediamenti Produttivi e Interazione con l'Ambiente (DIPIA), Rome, Italy
Search for more papers by this authorEnrico D'Emilia
Istituto Superiore per la Prevenzione E la Sicurezza del Lavoro (ISPESL), Dipartimento Insediamenti Produttivi e Interazione con l'Ambiente (DIPIA), Rome, Italy
Search for more papers by this authorCorresponding Author
Settimio Grimaldi
Istituto di Neurobiologia e Medicina Molecolare (INeMM), CNR, Rome, Italy
Istituto di Neurobiologia e Medicina Molecolare–Consiglio Nazionale delle Ricerche, Via Fosso del Cavaliere 100, 00133 Rome, Italy.Search for more papers by this authorAbstract
This work concerns the effect of low frequency electromagnetic fields (ELF) on biochemical properties of human oral keratinocytes (HOK). Cells exposed to a 2 mT, 50 Hz, magnetic field, showed by scanning electron microscopy (SEM) modification in shape and morphology; these modifications were also associated with different actin distribution, revealed by phalloidin fluorescence analysis. Moreover, exposed cells had a smaller clonogenic capacity, and decreased cellular growth. Indirect immunofluorescence with fluorescent antibodies against involucrin and β-catenin, both differentiation and adhesion markers, revealed an increase in involucrin and β-catenin expression. The advance in differentiation was confirmed by a decrease of expression of epidermal growth factor (EGF) receptor in exposed cells, supporting the idea that exposure to electromagnetic field carries keratinocytes to higher differentiation level. These observations support the hypothesis that 50 Hz electromagnetic fields may modify cell morphology and interfere in differentiation and cellular adhesion of normal keratinocytes. Bioelectromagnetics 25:118–126, 2004. © 2004 Wiley-Liss, Inc.
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