Research Article
Identification of transitional type II B cells in the salivary glands of patients with Sjögren's syndrome
Capucine Daridon,
Jacques-Olivier Pers,
Valérie Devauchelle,
Christine Martins-Carvalho,
Pascal Hutin,
Yvon-Louis Pennec,
Alain Saraux,
Pierre Youinou,
Capucine Daridon
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorJacques-Olivier Pers
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorValérie Devauchelle
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorChristine Martins-Carvalho
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorPascal Hutin
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorYvon-Louis Pennec
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorAlain Saraux
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorCorresponding Author
Pierre Youinou
Brest University Medical School Hospital, Brest, France
Laboratory of Immunology, Brest University Medical School Hospital, BP824, F-29609 Brest, FranceSearch for more papers by this authorCapucine Daridon,
Jacques-Olivier Pers,
Valérie Devauchelle,
Christine Martins-Carvalho,
Pascal Hutin,
Yvon-Louis Pennec,
Alain Saraux,
Pierre Youinou,
Capucine Daridon
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorJacques-Olivier Pers
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorValérie Devauchelle
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorChristine Martins-Carvalho
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorPascal Hutin
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorYvon-Louis Pennec
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorAlain Saraux
Brest University Medical School Hospital, Brest, France
Search for more papers by this authorCorresponding Author
Pierre Youinou
Brest University Medical School Hospital, Brest, France
Laboratory of Immunology, Brest University Medical School Hospital, BP824, F-29609 Brest, FranceSearch for more papers by this authorAbstract
Objective
To identify B cell subpopulations participating in the lymphocyte infiltrate of salivary glands from patients with primary Sjögren's syndrome. A special emphasis was placed on those B lymphocytes included in the ectopic germinal centers (GCs).
Methods
The presence of B cells in salivary glands and their polyclonality were ascertained by phenotyping and reverse transcription–polymerase chain reaction in salivary gland samples from 18 patients. Their phenotype was thoroughly analyzed using a number of double-staining combinations. The results obtained in tissue sections were confirmed by fluorescence-activated cell sorting analysis of B cells eluted from salivary glands, and these findings were compared with those in tonsils.
Results
Memory-type B cells were defined as CD20+,CD27+ and were seen in all specimens, whereas GCs were found in only 7 specimens. Furthermore, B cells found in these GCs lacked certain characteristics of centroblasts and centrocytes. Instead, they fulfilled the criteria for transitional type II (TII) B cells and resembled marginal-zone B cells. BAFF (the assistance of which is required for proper transformation of transitional TI B cells into transitional TII B cells) accumulated adjacent to transitional and marginal-zone–like B lymphocytes. Further evidence for the involvement of BAFF came from the expression of its receptors on infiltrating B cells.
Conclusion
These transitional TII and marginal-zone–like B cells are probably instrumental in the local production of autoantibodies and possibly influential in the ensuing destruction of epithelial cells.
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