Antigenicity of a recombinant Ro (SS-A) fusion protein
Judith A. James BS
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section, University of Oklahoma HSC, and former Sir Alexander Fleming Scholar
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorW. Daryl Dickey Md, Phd
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section, University of Oklahoma HSC, and Presbyterian Health Foundation Fellow
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorAtsushi Fujisaku Md, Phd
Arthritis and Immunology Program, Oklahoma Medical Research Foundation
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorCharles A. O'Brien BS
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, and the Department of Microbiology, University of Oklahoma HSC
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorSusan L. Deutscher PhD
Department of Microbiology, Duke University Medical Center
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorJack D. Keene PhD
Department of Microbiology, Duke University Medical Center
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorCorresponding Author
John B. Harley Md, Phd
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Rheumatology, Immunology, and Allergy Section, Department of Medicine, University of Oklahoma HSC, and the Oklahoma City VA Medical Center
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Oklahoma Medical Research Foundation, 825 Northeast 13th Street, Oklahoma City, OK 73104Search for more papers by this authorJudith A. James BS
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section, University of Oklahoma HSC, and former Sir Alexander Fleming Scholar
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorW. Daryl Dickey Md, Phd
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section, University of Oklahoma HSC, and Presbyterian Health Foundation Fellow
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorAtsushi Fujisaku Md, Phd
Arthritis and Immunology Program, Oklahoma Medical Research Foundation
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorCharles A. O'Brien BS
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, and the Department of Microbiology, University of Oklahoma HSC
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorSusan L. Deutscher PhD
Department of Microbiology, Duke University Medical Center
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorJack D. Keene PhD
Department of Microbiology, Duke University Medical Center
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Search for more papers by this authorCorresponding Author
John B. Harley Md, Phd
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Rheumatology, Immunology, and Allergy Section, Department of Medicine, University of Oklahoma HSC, and the Oklahoma City VA Medical Center
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, the Digestive Diseases and Nutrition Section and the Rheumatology, Immunology, and Allergy Section, Departments of Medicine and Microbiology, University of Oklahoma Health Sciences Center, and the Veterans Administration Medical Center, Oklahoma City, Oklahoma, and the Department of Microbiology, Duke University Medical Center, Durham, North Carolina.
Oklahoma Medical Research Foundation, 825 Northeast 13th Street, Oklahoma City, OK 73104Search for more papers by this authorAbstract
The antigenicity of the 60-kd human Ro (SS-A) synthesized in vitro from its complementary DNA as a β-galactosidase fusion protein (β-gal—Ro) was evaluated by Western blotting. In this analysis, almost all the anti-Ro (SS-A)-positive sera that bound β-gal-Ro also bound affinity-purified 60-kd human Ro (SS-A) (P > 0.005). Three of the 27 anti-Ro (SS-A) precipitinpositive sera, however, did not show reactivity on Western blot analysis, which suggests that in some sera, antigenicity to Ro (SS-A) is destroyed by denaturation. Of the 22 sera that were reactive with β-gal-Ro, 2 were not reactive with affinity-purified human Ro (SS-A). Two serum samples that did not react with β-gal-Ro were also reactive with affinity-purified human Ro (SS-A). Nevertheless, except for a small percentage of Ro (SS-A) precipitin-positive sera, the frequency of antibody binding to the fusion protein was similar to the frequency of binding to the purified antigen in Western blots. Recombinant Ro (SS-A) antigen may therefore be valuable in the serologic evaluation of anti-Ro (SS-A) autoantibodies.
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