Anti-ige autoantibodies in systemic lupus erythematosus
Corresponding Author
Barry L. Gruber MD
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Division of Rheumatology, Health Sciences Center, Stony Brook, NY 11794–8161Search for more papers by this authorLee D. Kaufman MD
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Search for more papers by this authorMary J. Marchese BA
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Search for more papers by this authorWilliam Roth PhD
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Search for more papers by this authorAllen P. Kaplan MD
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Search for more papers by this authorCorresponding Author
Barry L. Gruber MD
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Division of Rheumatology, Health Sciences Center, Stony Brook, NY 11794–8161Search for more papers by this authorLee D. Kaufman MD
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Search for more papers by this authorMary J. Marchese BA
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Search for more papers by this authorWilliam Roth PhD
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Search for more papers by this authorAllen P. Kaplan MD
Northport Veterans Administration Hospital and the State University of New York at Stony Brook, Division of Allergy, Rheumatology, and Clinical Immunology, Department of Medicine, Health Sciences Center, Stony Brook, New York.
Search for more papers by this authorAbstract
IgG and IgM autoantibodies directed against IgE were determined in 95 serum samples from 67 patients with systemic lupus erythematosus, by an enzyme-linked immunosorbent assay. IgM anti-IgE autoantibodies were found in 18 patients (27%) and IgG anti-IgE autoantibodies were detected in 23 patients (34%). The specificity of the immunoassay was confirmed by the ability to inhibit binding with IgE myeloma, but not other immunoglobulin isotypes and the demonstration that the reactivity was directed to the Fc ε fragment of IgE. The IgG fraction of certain sera with anti-IgE activity was capable of inducing histamine release from control basophils and cutaneous mast cells. Clinical associations with the presence of anti-IgE activity were sought by retrospective chart analysis of 61 patients. Significant correlation was found with articular involvement, lymphadenopathy, and anti-DNA antibodies (P < 0.05). Anti-IgE autoantibodies are observed in a significant number of patients with systemic lupus erythematosus and may contribute to the pathogenesis of the vascular and articular lesions characteristic of this disease.
Reference
- 1 Nawata Y, Koike T, Yanagisawa T, Iwamoto I, Itaya T, Yoshida S, Tomioka H: Anti-IgE autoantibody in patients with bronchial asthma. Clin Exp Immunol 58: 348–356, 1984
- 2 Nawata Y, Koike T, Hosokawa H, Tomioka H, Yoshida S: Anti-IgE autoantibody in patients with atopic dermatitis. J Immunol 135: 478–483, 1985
- 3 Williams RC Jr, Griffiths RW, Emmons JD, Field RC: Naturally occurring human antiglobulins with specificity for IgE. J Clin Invest 51: 955–967, 1972
- 4 Inganas M, Johansson SGO, Bennich H: Anti-IgE antibodies in human serum: occurrence and specificity. Int Arch Allergy Appl Immunol 65: 51–55, 1981
- 5 Carini C, Brostoff J: An antiglobulin: IgG anti-IgE: occurrence and specificity. Ann Allergy 51: 251–253, 1983
- 6 Quinti I, Brozek C, Wood N, Geha R, Leung OYM: Circulating IgG autoantibodies to IgE in atopic syndromes. J Allergy Clin Immunol 77: 586–589, 1986
- 7 Magnusson CGM, Vaerman JP: Autoantibodies of the IgM class against a human myeloma protein IgE (DES). I. Occurrence. Int Arch Allergy Appl Immunol 79: 149–155, 1986
- 8 Christian CL: Immune complex disease. N Engl J Med 280: 878–884, 1969
- 9 Koffler D, Agnello V, Kunkel HG: Polynucleotide immune complexes in serum and glomeruli of patients with systemic lupus erythematosus. Am J Pathol 74: 109–115, 1974
- 10 Biesecker G, Lavin L, Ziskind W, Koffler D: Cutaneous localization of membrane attack complex in discoid and systemic lupus erythematosus. N Engl J Med 306: 264–270, 1982
- 11 Tan EM, Cohen AS, Fries JF, Masi AT, McShane DJ, Rothfield NF, Shaller JG, Talal N, Winchester RJ: The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 25: 1271–1277, 1982
- 12 Gruber BL, Baeza M, Marchese MJ, Agnello V, Kaplan AP: Prevalence and functional role of anti-IgE autoantibodies in urticarial syndromes. J Invest Dermatol 90: 213–217, 1988
- 13 Lichtenstein LM, Osler AG: Studies on the mechanism of hypersensitivity phenomenon. IX. Histamine release from human leukocyte by ragweed pollen antigens. J Exp Med 120: 507–517, 1964
- 14 Beaven MA, Jacobsen S, Horakova Z: Modification of the enzymatic isotopic assay of histamine and its application of measurement of histamine in tissues, serum and urine. Clin Chim Acta 37: 91–96, 1972
- 15 Norman PS: Skin testing, Manual of Clinical Immunology. Second edition. Edited by NR Rose, H Friedman. Washington, DC, American Society for Microbiology, 1980
- 16 Sokol RR, Rohlf FJ: Biometry. San Francisco, WH Freeman, 1981, p 152
- 17 Magnusson CGM, Vaerman JP: Autoantibodies of the IgM class against a human myeloma IgE (DES). II. Specificity. Int Arch Allergy Appl Immunol 79: 157–161, 1986
- 18 Solley GO, Gleich GJ, Jordan RE, Schroeter AL: The late phase of the immediate wheal and flare skin reaction: its dependence upon IgE antibodies. J Clin Invest 58: 408–420, 1976
- 19 Kniker WT, Cochrane CG: Pathogenic factors in vascular lesions of experimental serum sickness. J Exp Med 122: 83–98, 1965
- 20 Baehr G, Klemperer P, Schrifrin A: A diffuse disease of the peripheral circulation usually associated with lupus erythematosus and endocarditis. Trans Assoc Am Physicians 50: 139–155, 1935
- 21 Couser WG, Salant DJ: In situ immune complex formation and glomerular injury. Kidney Int 17: 1–13, 1980
- 22 Koffler D, Agnello V, Winchester R, Kunkel HG: Systemic lupus erythematosus: prototype of immune complex nephritis in man (abstract). J Exp Med 134 (suppl): 169S, 1971
- 23 Majno G, Shea SM, Leventhal M: Endothelial contraction induced by histamine type mediators: an electron microscopic study. J Cell Biol 42: 647–672, 1969
- 24 Benveniste J, Egido J, Gutierrey Millef V: Evidence for the involvement of the IgE basophil system in acute serum sickness of rabbits. Clin Exp Immunol 26: 449–456, 1976
- 25 Kniker WT, Cochrane CG: The localization of circulating immune complexes in experimental serum sickness: the role of vasoactive amines and hydrodynamic forces. J Exp Med 127: 119–130, 1968
- 26 Cochrane CG: Mechanisms involved in the deposition of immune complexes (abstract). J Exp Med 134 (suppl): 75S, 1971
- 27 Braverman IM, Yen BS: Demonstration of immune complexes in spontaneous and histamine-induced lesions and in normal skin of patients with leukocytoclas-tic angiitis. J Invest Dermatol 64: 105–112, 1975
- 28 McPhaul JJ, Newcomb RW, Mullins JD, Thompson AL, Lordon RL, Rogers PW: Participation of immunoglobulin E in immune-mediated glomerulonephritis. Kidney Int 5: 292–299, 1974
- 29 Egido J, Sanchez Crespo M, Lahoz C, Garcia R, Lopez-Trascasa M, Hernando L: Evidence of an immediate hypersensitivity mechanism in systemic lupus erythematosus. Ann Rheum Dis 39: 312–317, 1980
- 30 Godfrey HP, Ilardi C, Engber W, Graziano FM: Quantitation of human synovial mast cells in rheumatoid arthritis and other rheumatic disease. Arthritis Rheum 27: 852–856, 1984
- 31 Gruber B, Poznansky M, Boss E, Partin J, Gorevic P, Kaplan AP: Characterization and functional studies of rheumatoid synovial mast cells: activation by secretagogues, anti-IgE, and a histamine-releasing lymphokine. Arthritis Rheum 29: 944–955, 1986
- 32 Malone DG, Irani A-M, Schwartz LB, Barrett KE, Metcalfe DD: Mast cell numbers and histamine levels in synovial fluids from patients with diverse arthritides. Arthritis Rheum 29: 956–963, 1986
