Synovial fluid exoglycosidases are predictors of rheumatoid arthritis and are effective in cartilage glycosaminoglycan depletion
Anna Polgár
National Institute of Rheumatology and Physiotherapy, Budapest, Hungary
Search for more papers by this authorBéla Gömör
Polyclinic of Hospitaller Brothers of St. John of God, Budapest, Hungary
Search for more papers by this authorPál Géher
Polyclinic of Hospitaller Brothers of St. John of God, Budapest, Hungary
Search for more papers by this authorTamás Lakatos
Polyclinic of Hospitaller Brothers of St. John of God, Budapest, Hungary
Search for more papers by this authorTibor T. Glant
Rush University at Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois
Search for more papers by this authorRenate E. Gay
University Hospital of Zurich, Zurich, Switzerland
Search for more papers by this authorCorresponding Author
Edit I. Buzás
Semmelweis University, Budapest, Hungary
Department of Genetics, Cell and Immunobiology, Semmelweis University, 4 Nagyvárad tér, H-1089 Budapest, HungarySearch for more papers by this authorAnna Polgár
National Institute of Rheumatology and Physiotherapy, Budapest, Hungary
Search for more papers by this authorBéla Gömör
Polyclinic of Hospitaller Brothers of St. John of God, Budapest, Hungary
Search for more papers by this authorPál Géher
Polyclinic of Hospitaller Brothers of St. John of God, Budapest, Hungary
Search for more papers by this authorTamás Lakatos
Polyclinic of Hospitaller Brothers of St. John of God, Budapest, Hungary
Search for more papers by this authorTibor T. Glant
Rush University at Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois
Search for more papers by this authorRenate E. Gay
University Hospital of Zurich, Zurich, Switzerland
Search for more papers by this authorCorresponding Author
Edit I. Buzás
Semmelweis University, Budapest, Hungary
Department of Genetics, Cell and Immunobiology, Semmelweis University, 4 Nagyvárad tér, H-1089 Budapest, HungarySearch for more papers by this authorAbstract
Objective
To analyze enzymes involved in joint damage by simultaneous investigation of glycosidases and matrix metalloproteinases (MMPs) in patients with various joint diseases.
Methods
Activities of glycosidases (β-D-glucuronidase, β-D-N-acetyl-glucosaminidase, β-D-N-acetyl-galactosaminidase, β-D-galactosidase, and α-D-mannosidase) were tested at an acidic pH as well as at the original pH of the synovial fluid (SF) samples in parallel with activities of MMP-1 and MMP-9.
Results
Patients with rheumatoid arthritis (RA) were characterized by significantly elevated activities of β-D-glucuronidase and β-D-N-acetyl-glucosaminidase in SF compared with patients with osteoarthritis, seronegative spondylarthritis, or acute sports injury. To select the best predictor for distinguishing among patient groups, a stepwise logistic regression analysis was performed; the strongest association was found to be between RA and β-D-glucuronidase/β-D-N-acetyl-glucosaminidase activities (measured at the pH of the SF). Further, a significant correlation was observed between the activity of SF β-D-N-acetyl-glucosaminidase and the level of rheumatoid factor. In vitro digestion of human hyaline cartilage samples revealed that the dominant glycosidases, alone or in combination with MMPs, proved to be effective in depleting glycosaminoglycans (GAGs) from cartilage.
Conclusion
These results suggest that exoglycosidases, which are present in the SF of RA patients, may contribute to the depletion of GAGs from cartilage and thereby facilitate the invasion of synovial cells and their attachment to cartilage in RA.
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