Knockdown of β-N-acetylglucosaminidase gene disrupts molting process in Heortia vitessoides Moore
Zihao Lyu
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorJingxiang Chen
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorZhixing Li
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorJie Cheng
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorChunyan Wang
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorCorresponding Author
Tong Lin
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Correspondence Tong Lin, College of Forestry and Landscape Architecture, South China Agricultural University, Room 607, 483 Wushan Road, Guangdong Province, Guangzhou 510642, China. Email: [email protected]
Search for more papers by this authorZihao Lyu
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorJingxiang Chen
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorZhixing Li
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorJie Cheng
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorChunyan Wang
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Search for more papers by this authorCorresponding Author
Tong Lin
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China
Correspondence Tong Lin, College of Forestry and Landscape Architecture, South China Agricultural University, Room 607, 483 Wushan Road, Guangdong Province, Guangzhou 510642, China. Email: [email protected]
Search for more papers by this authorAbstract
β-N-acetylglucosaminidase (NAG) is a key enzyme in insect chitin metabolism and plays an important role in many physiological activities of insects. The HvNAG1 gene was identified from the Heortia vitessoides Moore (Lepidoptera: Crambidae) cDNA library and its expression patterns were determined using quantitative real-time polymerase chain reaction. The results indicated that HvNAG1 mRNA levels were high in the midgut and before molting, and 20E could induce its expression. Subsequently, the HvNAG1 gene was knocked down via RNA interference to identify its functions. We found that 3 μg of dsNAG1 resulted in optimal interference at 48 and 72 hr after injection, causing a decrease in NAG1 protein content, which resulted in abnormal or lethal phenotypes, and a sharp decrease in the survival rate. These results indicate that HvNAG1 plays a key role in the molting process of H. vitessoides. However, the silencing of HvNAG1 had no significant effect on the chitin metabolism-related genes tested in this study. Our present study provides a reference for further research on the utility of key genes involved in the chitin metabolic pathway in the insect molting process.
CONFLICT OF INTERESTS
The authors declare that they have no conflict of interests.
Supporting Information
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|---|---|
| arch21561-sup-0001-NAG_table_S1.doc18 KB | Supporting information |
| arch21561-sup-0002-NAG_table_S2.doc31 KB | Supporting information |
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