Development and In Vitro Biological Evaluation of Hydrocolloid-Based Wound Dressings Incorporated With Capparis sepiaria Extract
Hlalisa Kula
Department of Chemistry, University of Fort Hare, Alice Campus, South Africa
Search for more papers by this authorShirley Motaung
Global Health and BioTech Pty, Pretoria, South Africa
Contribution: Conceptualization (lead), Data curation (lead), Funding acquisition (lead), Project administration (lead), Resources (lead), Supervision (lead), Validation (lead), Visualization (lead)
Search for more papers by this authorS. A. Adeyemi
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Data curation (lead), Formal analysis (lead), Investigation (lead), Methodology (lead), Resources (equal), Software (lead), Validation (lead), Visualization (lead), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorP. Ubanako
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Data curation (lead), Formal analysis (lead), Investigation (lead), Methodology (lead), Software (lead), Validation (equal), Visualization (equal), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorLindokuhle Ngema
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Data curation (lead), Formal analysis (lead), Investigation (equal), Methodology (equal), Resources (equal), Software (lead), Validation (lead), Visualization (lead), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorT. Y. Fonkui
Department of Biotechnology and Food Technology, University of Johannesburg, Johannesburg, South Africa
Contribution: Data curation (lead), Formal analysis (lead), Investigation (lead), Methodology (equal), Resources (lead), Validation (lead), Visualization (lead)
Search for more papers by this authorD. T. Ndinteh
Drug Discovery and Smart Molecules Research Labs, Centre for Natural Product Research, Department of Chemical Sciences, University of Johannesburg, Johannesburg, South Africa
Contribution: Conceptualization (lead), Formal analysis (lead), Funding acquisition (lead), Project administration (lead), Resources (lead), Software (lead), Supervision (lead), Validation (lead)
Search for more papers by this authorP. Kumar
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Conceptualization (lead), Data curation (equal), Formal analysis (lead), Funding acquisition (lead), Investigation (lead), Project administration (lead), Resources (lead), Software (lead), Visualization (lead), Writing - review & editing (lead)
Search for more papers by this authorY. E. Choonara
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Conceptualization (lead), Data curation (lead), Funding acquisition (lead), Project administration (lead), Resources (lead), Software (lead), Supervision (equal), Validation (lead), Visualization (lead), Writing - review & editing (lead)
Search for more papers by this authorCorresponding Author
Blessing A. Aderibigbe
Department of Chemistry, University of Fort Hare, Alice Campus, South Africa
Correspondence:
Blessing A. Aderibigbe ([email protected])
Contribution: Conceptualization (lead), Funding acquisition (lead), Project administration (lead), Resources (lead), Software (lead), Supervision (lead), Validation (lead), Visualization (lead), Writing - review & editing (lead)
Search for more papers by this authorHlalisa Kula
Department of Chemistry, University of Fort Hare, Alice Campus, South Africa
Search for more papers by this authorShirley Motaung
Global Health and BioTech Pty, Pretoria, South Africa
Contribution: Conceptualization (lead), Data curation (lead), Funding acquisition (lead), Project administration (lead), Resources (lead), Supervision (lead), Validation (lead), Visualization (lead)
Search for more papers by this authorS. A. Adeyemi
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Data curation (lead), Formal analysis (lead), Investigation (lead), Methodology (lead), Resources (equal), Software (lead), Validation (lead), Visualization (lead), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorP. Ubanako
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Data curation (lead), Formal analysis (lead), Investigation (lead), Methodology (lead), Software (lead), Validation (equal), Visualization (equal), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorLindokuhle Ngema
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Data curation (lead), Formal analysis (lead), Investigation (equal), Methodology (equal), Resources (equal), Software (lead), Validation (lead), Visualization (lead), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorT. Y. Fonkui
Department of Biotechnology and Food Technology, University of Johannesburg, Johannesburg, South Africa
Contribution: Data curation (lead), Formal analysis (lead), Investigation (lead), Methodology (equal), Resources (lead), Validation (lead), Visualization (lead)
Search for more papers by this authorD. T. Ndinteh
Drug Discovery and Smart Molecules Research Labs, Centre for Natural Product Research, Department of Chemical Sciences, University of Johannesburg, Johannesburg, South Africa
Contribution: Conceptualization (lead), Formal analysis (lead), Funding acquisition (lead), Project administration (lead), Resources (lead), Software (lead), Supervision (lead), Validation (lead)
Search for more papers by this authorP. Kumar
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Conceptualization (lead), Data curation (equal), Formal analysis (lead), Funding acquisition (lead), Investigation (lead), Project administration (lead), Resources (lead), Software (lead), Visualization (lead), Writing - review & editing (lead)
Search for more papers by this authorY. E. Choonara
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Contribution: Conceptualization (lead), Data curation (lead), Funding acquisition (lead), Project administration (lead), Resources (lead), Software (lead), Supervision (equal), Validation (lead), Visualization (lead), Writing - review & editing (lead)
Search for more papers by this authorCorresponding Author
Blessing A. Aderibigbe
Department of Chemistry, University of Fort Hare, Alice Campus, South Africa
Correspondence:
Blessing A. Aderibigbe ([email protected])
Contribution: Conceptualization (lead), Funding acquisition (lead), Project administration (lead), Resources (lead), Software (lead), Supervision (lead), Validation (lead), Visualization (lead), Writing - review & editing (lead)
Search for more papers by this authorFunding: This work was supported by the South African Medical Research Council, (SAMRC) and Technology and Human Resources for Industry Programme (THRIP).
ABSTRACT
Developing wound dressings containing plant extracts is an innovative method of preparing wound dressings suitable for treating infected wounds. Different species of Capparis are known for their rich sources of antioxidants with wound-healing effects. However, the Capparis sepiaria extracts have not been sufficiently explored for wound healing. Infected wounds are challenging to treat, revealing the need to design effective wound dressings. Novel single-layer hydrocolloids (SLHs) and double-layer hydrocolloids (DLHs) loaded with Capparis sepiaria aqueous extract were developed from carboxymethyl cellulose (CMC), sodium alginate (SA), and hydroxy methylcellulose (HPMC), and characterized using appropriate techniques. Their SEM images revealed an interwoven and porous morphology that promotes cell activities and gaseous exchange. The water vapor transmission rates and % porosity of the hydrocolloids ranged from 1000 ± 2.80 to 9500 ± 0.0025 g/m2/24 h and 70 ± 0.09 to 89%± 15.0%, respectively. The DLH2 hydrocolloids showed significant antibacterial efficacy against most bacterial strains, with MIC values of 15.625 μg/mL. The hemostatic effects of SLH3 and DLH3 were substantial, with the lowest absorbance values being 0.1869 and 0.1816, respectively. The prepared hydrocolloids were nontoxic, supported cellular migration suitable for accelerated wound healing, and exhibited antibacterial and hemostatic effects, making them promising dressings for treating infected and bleeding wounds.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available in the Supporting Information of this article.
Supporting Information
| Filename | Description |
|---|---|
| app56809-sup-0001-FigureS1.tifTIFF image, 3.3 MB |
Figure S1. FTIR spectra of hydrocolloids after biodegradation of SLH2, SLH7, and SLH9 at pH 5.5. |
| app56809-sup-0002-FigureS2.tifTIFF image, 2.4 MB |
Figure S2. FTIR spectra of hydrocolloids after biodegradation of SLH2, SLH7, and SLH9 at pH 7.4. |
| app56809-sup-0003-FigureS3.tifTIFF image, 2.8 MB |
Figure S3. FTIR spectra of hydrocolloids after biodegradation of DLH2 and DLH3 at pH 5.5. |
| app56809-sup-0004-FigureS4.tifTIFF image, 8.6 MB |
Figure S4. SEM images: (a) SLH2 at pH 5.5, (b) SLH7 at pH 5.5, (c) SLH2 at pH 7.4, (d) SLH7 at pH 7.4, (e) DLH2 at pH 5.5, and (f) DLH3 at pH 5.5. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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