A Fluid Multivalent Magnetic Interface for High-Performance Isolation and Proteomic Profiling of Tumor-Derived Extracellular Vesicles
Qi Niu
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
These authors contributed equally to this work.
Search for more papers by this authorDr. Yun Shu
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, China
These authors contributed equally to this work.
Search for more papers by this authorYuanqiang Chen
Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Soochow University, Suzhou, China
These authors contributed equally to this work.
Search for more papers by this authorDr. Zhi Huang
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
These authors contributed equally to this work.
Search for more papers by this authorZhixian Yao
Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Soochow University, Suzhou, China
Search for more papers by this authorDr. Xiaofeng Chen
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
Search for more papers by this authorFanghe Lin
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
Search for more papers by this authorJianzhou Feng
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, China
Search for more papers by this authorChen Huang
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Search for more papers by this authorDr. Hua Wang
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Search for more papers by this authorDr. Hongming Ding
Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Soochow University, Suzhou, China
Search for more papers by this authorProf. Chaoyong Yang
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
Search for more papers by this authorCorresponding Author
Dr. Lingling Wu
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Search for more papers by this authorQi Niu
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
These authors contributed equally to this work.
Search for more papers by this authorDr. Yun Shu
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, China
These authors contributed equally to this work.
Search for more papers by this authorYuanqiang Chen
Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Soochow University, Suzhou, China
These authors contributed equally to this work.
Search for more papers by this authorDr. Zhi Huang
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
These authors contributed equally to this work.
Search for more papers by this authorZhixian Yao
Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Soochow University, Suzhou, China
Search for more papers by this authorDr. Xiaofeng Chen
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
Search for more papers by this authorFanghe Lin
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
Search for more papers by this authorJianzhou Feng
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, China
Search for more papers by this authorChen Huang
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Search for more papers by this authorDr. Hua Wang
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Search for more papers by this authorDr. Hongming Ding
Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Soochow University, Suzhou, China
Search for more papers by this authorProf. Chaoyong Yang
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
Search for more papers by this authorCorresponding Author
Dr. Lingling Wu
Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Clinical Laboratory, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Search for more papers by this authorGraphical Abstract
A fluid multivalent magnetic interface was engineered in a microfluidic chip to improve the kinetics and thermodynamics of biomolecular recognition for efficient isolation of tumor-derived extracellular vesicles (T-EVs). With the assistance of magnetic and flow fields, this interface balanced affinity, selectivity, reversibility, and extendibility, enabling high-throughput recovery of T-EVs for protein profiling.
Abstract
Isolation and analysis of tumor-derived extracellular vesicles (T-EVs) are important for clinical cancer management. Here, we develop a fluid multivalent magnetic interface (FluidmagFace) in a microfluidic chip for high-performance isolation, release, and protein profiling of T-EVs. The FluidmagFace increases affinity by 105-fold with fluidity-enhanced multivalent binding to improve isolation efficiency by 13.9 % compared with a non-fluid interface. Its anti-adsorption property and microfluidic hydrodynamic shear minimize contamination, increasing detection sensitivity by two orders of magnitude. Moreover, its reversibility and expandability allow high-throughput recovery of T-EVs for mass spectrometric protein analysis. With the chip, T-EVs were detected in all tested cancer samples with identification of differentially expressed proteins compared with healthy controls. The FluidmagFace opens a new avenue to isolation and release of targets for cancer diagnosis and biomarker discovery.
Conflict of interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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