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CRISPR Craze Conquers the RNA World: Precise Manipulation of DNA and RNA Based on a Bacterial Defense System^†

Corresponding Author

Prof. Dr. Andrea Rentmeister

University of Münster, Institute of Biochemistry and Cells-in-Motion Cluster of Excellence (EXC1003-CiM), Wilhelm-Klemm-Strasse 2, 48149 Münster (Germany)

University of Münster, Institute of Biochemistry and Cells-in-Motion Cluster of Excellence (EXC1003-CiM), Wilhelm-Klemm-Strasse 2, 48149 Münster (Germany)Search for more papers by this author

Prof. Dr. Andrea Rentmeister,

Corresponding Author

Prof. Dr. Andrea Rentmeister

University of Münster, Institute of Biochemistry and Cells-in-Motion Cluster of Excellence (EXC1003-CiM), Wilhelm-Klemm-Strasse 2, 48149 Münster (Germany)

University of Münster, Institute of Biochemistry and Cells-in-Motion Cluster of Excellence (EXC1003-CiM), Wilhelm-Klemm-Strasse 2, 48149 Münster (Germany)Search for more papers by this author

First published: 27 February 2015

https://doi.org/10.1002/anie.201500563

Citations: 7

^†

A R. acknowledges financial support by the Deutsche Forschungsgemeinschaft within the Emmy Noether-Programme (RE 2796/2-1) and within the Cells in Motion-Cluster of Excellence (DFG EXC 1003).

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Graphical Abstract

It cuts both ways: The CRISPR/Cas9 genome engineering system has been extended from DNA to RNA. The sequence-specific targeting and cleavage of RNA can be achieved with S. pyogenes Cas9–gRNA if an appropriate short single-stranded oligonucleotide, the “PAMmer”, is added. This technique could lead to a general approach for sequence-specific RNA manipulation.

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Volume54, Issue16

April 13, 2015

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CRISPR Craze Conquers the RNA World: Precise Manipulation of DNA and RNA Based on a Bacterial Defense System^†

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CRISPR Craze Conquers the RNA World: Precise Manipulation of DNA and RNA Based on a Bacterial Defense System†

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CRISPR Craze Conquers the RNA World: Precise Manipulation of DNA and RNA Based on a Bacterial Defense System^†