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Structural Basis for Regulation of Ribosomal RNA 2′-O-Methylation†
†
H.S. and M.H. are supported by DFG (SFB902). H.S. is a member of the DFG-funded Cluster of Excellence: Macromolecular Complexes. BMRZ is supported by the State of Hesse.
Graphical Abstract
Methods that interrogate different, yet overlapping structural-size ranges were integrated to gain high-resolution information on the Pyrococcus furiosus box C/D ribonucleoprotein complex. The new structure shows how 2′-O-methylation of RNA substrates through four active methyltransferases occurs in a strictly sequential manner.
