Acetylcholinesterase-Catalyzed Hydrolysis Allows Ultrasensitive Detection of Pathogens with the Naked Eye†
Dingbin Liu
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorZhantong Wang
National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102 (China)
Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361005 (China)
Search for more papers by this authorDr. Albert Jin
Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20982 (USA)
Search for more papers by this authorXinglu Huang
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorXiaolian Sun
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorFu Wang
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorQiang Yan
National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102 (China)
Search for more papers by this authorShengxiang Ge
National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102 (China)
Search for more papers by this authorProf. Ningshao Xia
National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102 (China)
Search for more papers by this authorGang Niu
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorGang Liu
Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361005 (China)
Search for more papers by this authorDr. A. R. Hight Walker
Optical Technology Division, Physics Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 (USA)
Search for more papers by this authorCorresponding Author
Dr. Xiaoyuan Chen
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)Search for more papers by this authorDingbin Liu
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorZhantong Wang
National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102 (China)
Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361005 (China)
Search for more papers by this authorDr. Albert Jin
Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20982 (USA)
Search for more papers by this authorXinglu Huang
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorXiaolian Sun
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorFu Wang
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorQiang Yan
National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102 (China)
Search for more papers by this authorShengxiang Ge
National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102 (China)
Search for more papers by this authorProf. Ningshao Xia
National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102 (China)
Search for more papers by this authorGang Niu
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Search for more papers by this authorGang Liu
Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361005 (China)
Search for more papers by this authorDr. A. R. Hight Walker
Optical Technology Division, Physics Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 (USA)
Search for more papers by this authorCorresponding Author
Dr. Xiaoyuan Chen
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892 (USA)Search for more papers by this authorThis work was supported in part the Major State Basic Research Development Program of China (973 Program; 2013CB733802 and 2014CB744503), the National Science Foundation of China (NSFC; 81101101, 51273165, 81201086, 81201190, and 81371596), a Key Project of the Chinese Ministry of Education (212149), the Intramural Research Program (IRP) of the National Institute of Biomedical Imaging and Bioengineering (NIBIB), and the National Institutes of Health (NIH). D.L. was supported by a postdoctoral fellowship from NIH-NIBIB/NIST NRC.
Graphical Abstract
Seeing is believing: A rapid diagnostic platform for pathogen detection based on the acetylcholinesterase-catalyzed hydrolysis reaction has been developed. Owing to signal amplification strategies, the sensitivity of this assay is comparable to that of PCR. In addition, the readout of the assay is based on solution color change, which can be easily observed by the naked eye alone.
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