Convergent Chemical Synthesis of Histone H2B Protein for the Site-Specific Ubiquitination at Lys34†
Peter Siman
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105 (Israel) http://www.bgu.ac.il/∼abrik
These authors contributed equally to this work.
Search for more papers by this authorDr. Subramanian Vedhanarayanan Karthikeyan
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105 (Israel) http://www.bgu.ac.il/∼abrik
These authors contributed equally to this work.
Search for more papers by this authorDr. Miroslav Nikolov
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Göttingen 37077 (Germany)
Search for more papers by this authorDr. Wolfgang Fischle
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Göttingen 37077 (Germany)
Search for more papers by this authorCorresponding Author
Prof. Ashraf Brik
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105 (Israel) http://www.bgu.ac.il/∼abrik
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105 (Israel) http://www.bgu.ac.il/∼abrikSearch for more papers by this authorPeter Siman
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105 (Israel) http://www.bgu.ac.il/∼abrik
These authors contributed equally to this work.
Search for more papers by this authorDr. Subramanian Vedhanarayanan Karthikeyan
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105 (Israel) http://www.bgu.ac.il/∼abrik
These authors contributed equally to this work.
Search for more papers by this authorDr. Miroslav Nikolov
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Göttingen 37077 (Germany)
Search for more papers by this authorDr. Wolfgang Fischle
Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Göttingen 37077 (Germany)
Search for more papers by this authorCorresponding Author
Prof. Ashraf Brik
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105 (Israel) http://www.bgu.ac.il/∼abrik
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105 (Israel) http://www.bgu.ac.il/∼abrikSearch for more papers by this authorThis work was supported by the Israel Science Foundation (A.B.) and the Max Planck Society (W.F.). P.S. gratefully acknowledges the Israel Ministry of Science and Technology for financial support.
Graphical Abstract
As You Like It: The convergent chemical synthesis of the histone H2B protein was achieved. This approach enabled the attachment of ubiquitin at Lys34 (see picture) and should make it possible to synthesize H2B with any other posttranslational modification in demand.
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