Light-Regulated Stapled Peptides to Inhibit Protein–Protein Interactions Involved in Clathrin-Mediated Endocytosis†
Dr. Laura Nevola
Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
These authors contributed equally to this work.
Search for more papers by this authorAndrés Martín-Quirós
Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
These authors contributed equally to this work.
Search for more papers by this authorDr. Kay Eckelt
Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
Search for more papers by this authorDr. Núria Camarero
Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
Search for more papers by this authorDr. Sébastien Tosi
Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
Search for more papers by this authorDr. Artur Llobet
Bellvitge Biomedical Research Institute (IDIBELL) (Spain)
Search for more papers by this authorCorresponding Author
Prof. Dr. Ernest Giralt
Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
University of Barcelona (Spain)
Ernest Giralt, Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
Pau Gorostiza, Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
Search for more papers by this authorCorresponding Author
Prof. Dr. Pau Gorostiza
Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
CIBER-BBN and ICREA (Spain)
Ernest Giralt, Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
Pau Gorostiza, Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
Search for more papers by this authorDr. Laura Nevola
Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
These authors contributed equally to this work.
Search for more papers by this authorAndrés Martín-Quirós
Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
These authors contributed equally to this work.
Search for more papers by this authorDr. Kay Eckelt
Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
Search for more papers by this authorDr. Núria Camarero
Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
Search for more papers by this authorDr. Sébastien Tosi
Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
Search for more papers by this authorDr. Artur Llobet
Bellvitge Biomedical Research Institute (IDIBELL) (Spain)
Search for more papers by this authorCorresponding Author
Prof. Dr. Ernest Giralt
Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
University of Barcelona (Spain)
Ernest Giralt, Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
Pau Gorostiza, Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
Search for more papers by this authorCorresponding Author
Prof. Dr. Pau Gorostiza
Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
CIBER-BBN and ICREA (Spain)
Ernest Giralt, Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028 Barcelona (Spain)
Pau Gorostiza, Institute for Bioengineering of Catalonia (IBEC), C/Baldiri Reixac 15-21, 08028 Barcelona (Spain)
Search for more papers by this authorWe are grateful to A. Adeva for help with peptide synthesis at the initial stages of the project, to A. Lladó and L. Bardia for technical support with cell imaging and useful discussions, and to CCiTUB Flow Cytometry Facility for technical support. We thank S. J. Royle for providing the LCa–mRFP plasmid, H. McMahon for the β-appendage plasmid, T. Kirchhausen for the BSC-1 cell line. We acknowledge financial support from the Human Frontier Science Program through a Career Development Award (CDA022/2006), from the European Research Council through the “Opticalbullet” Starting Grant (ERC-StG-210355/2007), from the European Commission through the “Focus” ICT-FET grant (FP7-ICT-2009-270483), from the Ministry of Education through grant CTQ2008-06160, and a FPU fellowship (to A.M.-Q.), and from the RecerCaixa and Marató de TV3 foundations.
Graphical Abstract
Control of membrane traffic: Photoswitchable inhibitors of protein–protein interactions were applied to photoregulate clathrin-mediated endocytosis (CME) in living cells. Traffic light (TL) peptides acting as “stop” and “go” signals for membrane traffic can be used to dissect the role of CME in receptor internalization and in cell growth, division, and differentiation.
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